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白藜芦醇抑制H460肺癌细胞的增殖作用研究

, PP. 12-15

Keywords: mi-622,白藜芦醇,肺癌

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Abstract:

目的探讨白藜芦醇对肺癌细胞H460增殖的影响,以探索小分子核酸miR-622在白藜芦醇抗癌中的作用。方法用不同浓度的白藜芦醇处理肺癌细胞H460,利用CCK8试剂盒检测细胞增殖,以miRNA定量试剂盒检测miR-622的表达水平。利用Lipofectamine2000将miR-622模拟物、miR-622抑制剂及其相应对照转染进入H460细胞内。结果50μmol/L白藜芦醇处理48h即可使H460的细胞增殖明显降低至(49.77±1.33)%,差异有统计学意义(P结论白藜芦醇可抑制H460细胞的生长,且这种作用与miR-622的表达上调密切相关。

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