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克雷霉素、头孢噻肟对艰难梭菌毒素转录和表达的影响

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Keywords: 艰难梭菌,克雷霉素,头孢噻肟,B毒素

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Abstract:

目的研究1/2最小抑菌浓度(MIC)剂量的克雷霉素和头孢噻肟对中国艰难梭菌A-B+高分离株BJ08的生长、毒力基因转录和毒素分泌的影响。方法BJ08菌株分别生长在含有1/2MIC的克雷霉素或头孢噻肟以及无抗生素的脑心浸液肉汤(BHI)液体培养基中。每4个小时,通过检测其OD值以确定其生长曲线;通过实时荧光定量PCR检测其毒力基因的表达;通过酶联免疫吸附测定法(ELISA)检测上清和细胞内B毒素的表达。结果BJ08在1/2MIC剂量的克雷霉素存在的情况下,菌株的生长和毒素表达几乎不受影响;但是在1/2MIC剂量的头孢噻肟存在的情况下,菌株生长变慢,到达对数期的时间延迟;毒力基因和B毒素的表达提前且增加。结论实验证明在亚MIC剂量的不同的抗生素存在的情况下对中国株A-B+艰难梭菌的生长和毒力基因表达影响不同。我们的研究对于临床医师规范治疗CDAD和合理应用抗生素有一定的参考价值。

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