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宫颈病变中FHIT基因微卫星不稳定性的研究

, PP. 320-322

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Abstract:

目的通过对宫颈病变中脆性组氨酸三联体(Fragilehistidinetraid,FHIT)基因上三个微卫星位点D3S1300、D3S1234和D3S1481的微卫星不稳定性(Microsatelliteinstability,MSI)分析,探讨其与宫颈癌的发生、发展的关系。方法采用聚合酶链反应-非变性聚丙烯酰胺凝胶电泳-溴化乙锭染色技术检测FHIT基因中3个微卫星位点D3S1300、D3S1234和D3S1481的MSI。结果MSI在宫颈上皮内瘤样病变(Cervicalintraepithelialneoplasia,CIN)组与宫颈癌组检出率差异有统计学意义。在宫颈癌中I期与II期差异无统计学意义,与III~IV期比较差异有统计学意义,MSI的发生率与临床分期有相关,与病理分级无关。结论MSI在宫颈癌中的检出率远远高于CIN,表明MSI在宫颈癌发展过程中起重要作用,是宫颈癌恶性进展中的早期事件。

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