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p21Ras对哮喘大鼠气道平滑肌细胞增殖的影响

, PP. 271-274

Keywords: 气道平滑肌细胞,p21Ras,哮喘,细胞周期

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Abstract:

目的观察p21Ras对哮喘大鼠气道平滑肌细胞增殖的影响。方法14只SD大鼠,随机分为哮喘组和对照组,建立大鼠哮喘模型,采用酶消化法培养气道平滑肌细胞,免疫组织化学鉴定为平滑肌细胞,透射电镜观察细胞形态,分别取第3代对照组及哮喘组细胞,各自分为以下4个处理组,即对照组分为a.对照空白组不加任何干预剂;b.对照干预组;哮喘组分为c.哮喘空白组;d.哮喘干预组。加入10mMFPTIII干预,流式细胞仪分析细胞周期分布,经Levene’s方差齐性检验,按α=0.05水准,4组总体方差齐,组间比较采用Bonferron法检验,结果通过p21Ras干预后,哮喘空白组G0/G1期气道平滑肌细胞细胞(AMSC)比例显著低于对照空白组及哮喘干预组(P结论p21Ras影响哮喘大鼠气道平滑肌细胞周期时相中G0/G1的比例,使其增殖受到抑制,提示p21Ras与哮喘气道平滑肌的增殖密切相关。

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