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建立实时定量PCR检测临床样品中的麻风杆菌DNA

, PP. 1172-1174

Keywords: 麻风病,实时定量PCR,组织液,皮损组织,血液

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Abstract:

目的探讨建立实时定量PCR检测麻风病的方法,评价该方法对于麻风病的诊断价值。方法用17种可培养的分枝杆菌和4种常见球菌和杆菌检测RT-PCR的特异性,并系列稀释已知量的菌液至10-9以检测RT-PCR的敏感性。临床标本40例,包括TT2,BT17,BL17,LL1,I3例。其中取皮损组织26份,常规组织液27份及血液样品34份。结果RT-PCR最低检测限约为4.9个麻风菌/反应,17种其他分枝杆菌和4种细菌常见球菌和杆菌DNA均未扩增出RLEP片段。检测皮损、组织液和血液三种标本,发现皮损标本中麻风菌量最多;BI值与皮损中麻风菌DNA的Ct值之间存在负性相关,提示RTPCR可作为参考值用于定量患者麻风菌载量。结论用RLEPRT-PCR定量麻风菌细菌负荷,该方法敏感性高、特异性强,具有临床参考价值。

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