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IgGFc基因增强乙型脑炎DNA疫苗细胞免疫效应的分析

DOI: 10.13604/j.cnki.46-1064/r.2015.10.02, PP. 1161-1164

Keywords: 日本脑炎病毒,DNA疫苗,IgG,Fc段,树突状细胞

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Abstract:

目的评价IgGFc段编码基因不同构建方式对乙型脑炎(JE)DNA疫苗细胞免疫应答的影响。方法以套式RT-PCR法获取BALB/c鼠IgGFc编码基因,构建含乙型脑炎病毒(JEV)prME蛋白与IgGFc编码基因以及单纯IgGFc编码基因重组质粒,分别命名pJME/IgGFc和pIgGFc,以不同免疫原肌注免疫BALB/c小鼠,经流式细胞仪检测不同免疫原免疫鼠后脾T淋巴细胞亚群及Th细胞内细胞因子(IFN-γ、IL-4)变化,乳酸脱氢酶(Lactatedehydrogenase,LDH)法测定细胞毒性T淋巴细胞(CytotoxicTlymphocytes,CTL)活性。数据进行单因素方差分析。结果pJME/IgGFc组CD4+T淋巴细胞比例为(66.64±5.84)%,明显高于pJME+pIgGFc、pJME、JE灭活疫苗和pcDNA3.1(+)组(P结论相对于pJME+pIgGFc联合免疫组,pJME/IgGFc融合质粒免疫组能够诱导更强的细胞免疫反应。

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