全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

肺结核患儿与卡介苗成功接种儿童外周血基因克隆研究

, PP. 838-840

Keywords: 儿童,肺结核,卡介苗,抗体,TCR,基因克隆

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的研究肺结核患儿与卡介苗接种抗体阳性儿童外周血TCRVβ基因克隆。方法分别提取15名肺结核患儿及15名卡介苗接种抗体阳性儿童外周血RNA,经逆转录扩增后,以Genescan方法分别检测其TCRVβ的基因克隆。以健康儿童卡介苗接种抗体阴性作为对照。结果肺结核患儿与卡介苗接种抗体阳性儿童外周血TCRVβ基因家族均以Vβ2,Vβ16,Vβ21,Vβ22克隆增生为主,克隆抑制以Vβ14和Vβ17为主;不同的个体之间TCRVβ基因克隆不尽相同。结论卡介苗接种抗体阳性儿童与肺结核患儿具有相似的TCRTCRVβ基因克隆增生家族。

References

[1]  Dongzhi Cen, Yangqiu Li, Shaohua Chen, Lijian Yang, Zhi Yu. Distribution and clonality of peripheral blood TCR Vαsubfamily T cells in patients with acute promyelocytic leukemia.[J].Chin-German J Clin Oncol.2007, 6(6): 591-593.
[2]  Assaf C, HummelM, Dippel E, et al. High detection rate of T-cell receptor beta chain rearrangements in T2cell lymphop roliferations by family specific polymerase chain reaction in combination with the GeneScan technique and DNA sequencing [J]. Blood, 2000, 96 (2): 640 - 646.
[3]  Zhou J, Ma R, Luo R, Sun Y, He X, Sun W, Tang W, Yao X. Primary exploration of molecular and spectratyping features of CDR3 of TCR β chain in the peripheral blood and tissue of patients with colorectal carcinoma[J]. Cancer Epidemiol, 2010, 34(6): 733-740.
[4]  Pnee J, Chevalier S, Preisser L, et al. Chronically inflame human tissues are inflatrated by highly differetiated Th17 lymphocytes[J]. J Immunol, 2008, 180(11): 7423-7430.
[5]  Avgustin B, Kotnik V, Skoberne M. CD69 expression on CD4+ T lymphocytes after in vitro stimulation with tuberculisis is an indicator of immune sensitization against mycobacterium tuberculosis amtigens[J]. Clin Diagn Lab Immunol, 2005, 12(1): 101-106.
[6]  Qiao D, Li L, Guo J, et al. Mycobacterium tuberculosis Culture Filtrate Protein 10-Specific Effector/Memory CD4+ and CD8+ T Cells in Tubercular Pleural Fluid, with Biased Usage of T Cell Receptor V(beta) Chains[J]. Infect Immun. 2011, 79(8): 3358-3365.
[7]  Dong L, Li P, Oenema T, et al. Public TCR use by herpes simplex virus-2-specific human CD8 CTLs[J]. J Immunol, 2010, 184(6): 3063-3071.
[8]  Jian-wei Zhou, Rui Ma, Wen-tai Tang, Rong Luo, Xin-sheng Yao. Primary exploration of the third complementarity determining region spectratyping and molecular features of T cell receptor alpha chain in the peripheral blood and tissue of patients with colorectal carcinoma[J]. ACTA Medica Mediterranea, 2011,27:97-104.
[9]  Ren GL, Chen JP, Jia MM,et al. T cell receptor diversity of CD8+ T lymphocytes and its association with viral load in individuals with HIV-1 infection[J].Chin J Prev.Med. 2009, 43(5): 404-408.
[10]  Akbulut HH, Deveci F, Celik I, Ilhan F, Turgut T. Investigation of T cell receptors in the peripheral blood of patients with active pulmonary tuberculosis[J]. Indian J Med Microbiol. 2009, 27:40-43.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133