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苯对细胞增殖抑制率的影响及保护因素探讨

, PP. 812-814

Keywords: 苯中毒,血清,氨磷汀,保护剂

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Abstract:

目的探讨苯对骨髓单个核细胞增殖抑制率的影响,同时了解血清及氨磷汀能否作为保护因素减轻苯对细胞的影响。方法实验分四组空白对照组、(0.25、3.5、20μmol/L)苯组、(0.25、3.5、20μmol/L)苯+(2、10、50μg/mL)氨磷汀组、(0.25、3.5、20μmol/L)苯+血清组,通过细胞增殖检测(CCK-8法)并计算骨髓单个核细胞增殖抑制率。结果高浓度苯组较低、中浓度苯组更能抑制细胞增殖(P0.05)。(0.25、3.5、20μmol/L)苯+(50、10、2μg/ml)氨磷汀,细胞增殖抑制率与相应浓度苯组比较,结果均具有显著性差异(P结论细胞增殖抑制率随苯剂浓度的增加而增加,苯毒性存在剂量依赖效应;血清及氨磷汀均能减轻苯对细胞的增殖毒性,可能可以作为苯中毒细胞的保护剂。

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