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HPVE6/E7mRNA检测在宫颈癌筛查中的应用

, PP. 1493-1495

Keywords: 人乳头瘤病毒,宫颈肿瘤,E6/E7mRNA,石蜡包埋,bDNA技术

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Abstract:

目的通过检测同一宫颈上皮内癌变或宫颈癌疑似病例宫颈石蜡包埋样本及脱落细胞中HPVE6/E7mRNA的水平,分析两种检测结果的差异,探讨二者在宫颈癌筛查的应用价值。方法对302例宫颈上皮内癌变或宫颈癌疑似病例分别取宫颈活检组织及脱落细胞,两类标本均采用bDNA技术进行HPVE6/E7mRNA检测,同时行病理学检查,并对检测的阳性率及灵敏度、特异度做出统计学分析。结果石蜡包埋组织检测HPVE6/E7mRNA的阳性率为48.7%,高于脱落细胞的阳性率40.1%(χ2=4.192,P2=5.046,P2=0.641,P>0.05)。结论检测石蜡包埋样本中的HPVE6/E7mRNA,可更加精确预测宫颈病变进展风险,对宫颈癌筛查工作具有更好的临床意义。

References

[1]  Sveinung Wergeland Sørbye, Silje Fismen, Tore Gutteberg,et al. Triage Of Women with Minor Cervical Lesions: Data Suggesting a ‘‘Test and Treat’’ Approach for HPV E6/E7 mRNA Testing [J].PLoS ONE, 2010 ,5(9):e12724.
[2]  E.A. Burger, H.Kornon, M.Klemp,et al. HPV mRNA tests for the detection of cervical intraepithelial neoplasia: A systematic review [J].Gynecologic Oncology, 2011 ,120(3):430-438.
[3]  Paola Cattani, Alessia Siddu, Sara D′Onghia,et al. 2009. RNA (E6/E7) assays versusDNA (E6/E7) assays for risk evaluation for women infected with humanpapillomavirus [J] .Clin. Microbiol,2009, 47(7):2136-2141.
[4]  王华,陈亚宝,叶丽,等.应用支链DNA技术检测人乳头瘤病毒E6/E7mRNA在宫颈疾病筛查中的价值 [J].中华临床医师杂志(电子版),2011, 5(15).
[5]  Robert D,Burk,Zigui Chen,Koenraad Van Doorslaer. Human Papillomaviruses: Genetic Basis of Carcinogenicity [J]. Public Health Genomics,2009,12(5-6):281-290.
[6]  Paola Cattani, Gian Franco Zannoni, Caterina Ricci, etal. Clinical Performance of Human- Papillomavirus E6 and E7 mRNA Testing for High-Grade Lesions of the Cervix [J].Clinical Microbiol Ogy, 2009, 47(12): 3895-3901.
[7]  Mody CA,Laimins LA. Human papillomavirus oncoproteins:Pathways to tansformation retrieved no results [J].Nat Rev Cancer,2010,10(8):550-560.
[8]  Adriana Vince, Snjezana Zidovec Lepej. Diagnostic methods and techniques in cervicai cancer prevention Part II: Molecular diagnostics of HPV infection [J].Med Glas ,2010,7(1):18-25 .
[9]  Yang W, Maqsodi B, Ma Y, et al.Direct quantification of gene expression in homogenates of formalin-fixed,paraffin-embedded tissue [J].Biotechniques,2006,40(5):481-486.
[10]  Siri Hovland, Susanne Muller, Hanne Skomedal,etal. E6/E7 mRNA expression analysis: A test for the objective assessment of cervical adenocarcinoma in clinical prognostic procedure [J].Internagtional Journal Of Oncology,2010,36(6): 1533-1539.

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