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珠海市2013年肠道病毒71型和柯萨奇病毒A16型基因特征

, PP. 1429-1434

Keywords: 手足口病,肠道病毒71型,柯萨奇病毒A组16型,基因型

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Abstract:

目的分析2013年珠海市手足口病(Hand-foot-mouthdisease,HFMD)患者中,肠道病毒71型(EV71)和柯萨奇病毒A组16型(CoxA16)的分子流行病学特征。方法采集珠海市2013年HFMD临床诊断病例粪便标本215份,选取其中RealtimeRT-PCR检测EV71病毒核酸阳性的18份和CoxA16病毒核酸阳性的8份标本,进行VP4区基因扩增,并进行核苷酸序列和遗传进化关系分析。结果16份EV71扩增测序成功,与C4a基因亚型代表株具有最高的核苷酸序列同源性(95.2%~99.5%)和氨基酸同源性(91.3%~100%);5份CoxA16扩增测序成功,有2份与B2a亚型代表株具有最高的核苷酸序列同源性(98.6%~99.0%)和氨基酸同源性(97.1%~98.5%),3份与B2b亚型代表株具有最高的核苷酸序列同源性(96.6%~97.1%)和氨基酸同源性(100%)。结论2013年珠海市流行的EV71属于C4a基因亚型,CoxA16为B2a和B2b两种基因亚型。

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