|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
重组柞蚕抗菌肽AD的纯化及鉴定
, PP. 15-18
Keywords: 抗菌肽AD,高效液相色谱,基质辅助激光解析—,飞行时间质谱,流式细胞术
Abstract:
目的从重组柞蚕抗菌肽AD毕赤酵母发酵液中分离纯化出抗菌肽纯品,并对其进行纯度、分子量和抗菌作用的鉴定。方法用离子交换和疏水层析法对发酵液中的重组柞蚕抗菌肽AD进行分离纯化,用高效液相色谱法分析产物的纯度,再用基质辅助激光解析—飞行时间质谱仪对产物进行分子量鉴定,并结合传统微量肉汤稀释法和流式细胞术分析重组柞蚕抗菌肽AD纯品对E.coli敏感株及耐药株的抗菌作用。结果重组柞蚕抗菌肽AD经离子交换和疏水层析纯化后的纯度达到98.40%,分子量为4068.15Da,与所设计的重组柞蚕抗菌肽AD大小相近。所纯化出的重组柞蚕抗菌肽AD对E.coli的敏感株ATCC25922和产β-内酰胺酶耐药株株ATCC35218的抗菌作用均为MIC8μg/mL,而头孢他啶和氨苄西林对耐药株ATCC35218的MIC的值分别为8μg/mL和>32μg/mL。结论成功纯化分离出具有自主知识产权的重组柞蚕抗菌肽AD,其对E.coli敏感株和耐药株具有相同的抗菌效果。
| [1] | 汪复, 朱德妹, 胡付品,等. 2008年中国CHINET细菌耐药性监测[J]. 中国感染与化疗杂志, 2009, 5: 321-329.
|
| [2] | McArthur AG,Waglechner N,Nizam F,et al. The comprehensive antibiotic resistance database [J]. Anti Chem,2013,57（7）:3348-3357.
|
| [3] | Dhillon RH, Clark J. ESBLs: A Clear and Present Danger? [J]. Crit Care Res Pract, 2012, 625170.
|
| [4] | Pushpanathan M;Gunasekaran P;Rajendhran J. Antimicrobial peptides: versatile biological properties [J]. Int J Pept,2013 ,675391.
|
| [5] | Pinheiro da Silva F;Machado MC . Antimicrobial peptides: clinical relevance and therapeutic implications [J].Peptides,2012,36(2 ):308-314.
|
| [6] | D'Costa VM, King CE, Kalan L, et a l. Antibiotic resistance is ancient [J]. Nature,2011,477（7365）：4574-61
|
| [7] | Bechara FG, Sand M, Skrygan M, et al. Acne inversa: evaluating antimicrobial peptides and proteins [J]. Ann Dermatol, 2012 , 24(4): 393-397.
|
| [8] | Li Y. Carrier proteins for fusion expression of antimicrobial peptides in Escherichia coli [J]. Biotechnol Appl Biochem, 2009, 54(1): 1-9.
|
| [9] | Yeung AT, Gellatly SL, Hancock RE. Multifunctional cationic host defence peptides and their clinical applications [J]. Cell Mol Life Sci, 2011, 68(13): 2161-2176.
|
| [10] | 黄自然, 黄亚东, 温刘发,等. 抗菌肽生物工程及其应用[J]. 蚕业科学, 2005, 31(4): 375-381.
|
| [11] | 邓平建, 房师松, 杨冬燕,等. 转抗菌肽CAD基因酵母饲料添加剂的安全性评价[J]. 卫生研究, 2004, 33(5): 565-568.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
