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OALib Journal期刊
ISSN: 2333-9721
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纳米钛酸锶钡对牛血清白蛋白的吸附行为
Abstract:
以硝酸钡、硝酸锶和钛酸四正丁酯为原料,采用柠檬酸络合溶胶-凝胶法制备纳米钛酸锶钡(nano-barium-strontiumtitanate,n-BST)粉体。用X射线衍射、透射电子显微镜和Fourier变换红外分析表征n-BST粉体的结构与性能。研究n-BST粉体对牛血清白蛋白(bovineserumalbumin,BSA)的吸附行为,考察吸附条件和洗脱条件,并分别用红外光谱和紫外光谱以及荧光光谱分析吸附作用和解吸过程对BSA结构的影响。结果表明当介质的pH=4.10时,n-BST对BSA具有很强的吸附能力,其吸附行为符合Langmuir吸附等温方程式和HO准二级动力学模型,吸附过程的焓变ΔH=-13.30kJ/mol,自由能变(ΔG)和熵变(ΔS)均为负值,吸附活化能Ea=16.18kJ/mol,该吸附过程是以氢键作用力为主的自发放热过程。被吸附的BSA可用pH=7.54的缓冲溶液完全洗脱。吸附过程和洗脱过程没有明显改变BSA的结构。
| [1] | MAO Chaoliang,DONG Xianlin,ZENG Tao,et al.Nonhydrolytic sol–gel synthesis and dielectric properties of ultrafine-grained and ho-mogenized Ba0.7Sr0.3TiO3[J].Ceram Int,2008,34(1):45–49. [2]JIANG Wanquan,GONG Xiuqing,CHEN Zuyao,et al.Preparation of barium strontium titanate Ba1·xSrxTiO3single-crystal nanorods by a novel combined method[J].Ultrasonics Sonochem,2007,14(2):208–212. [3]薛卫东,李言荣,杨春.BaxSr1–xTiO3精细结构的第一性原理研究[J].化学物理学报,2005,12(2):179–182.XUE Weidonga,LI Yanrong,YANG Chu.Chin J Chem Phys(in Chi-nese),2005,12(2):179–182. [4]苏会东,张东.纳米钛酸锶钡粉体制备及其对水中Cd～(2+)吸附行为[J].化工学报,2006,57:2892–2896.SU Huidong,ZHANG Dong.J Chem Ind Eng(in Chinese),2006,57:2892–2896. [5]张东,苏会东,高虹.钛酸锶钡纳米粉体的制备及其对铬(Ⅵ)和铬(Ⅲ)吸附性能[J].冶金分析,2007,27(4):7–10.ZHANG Dong,SU Huidong,GAO Hong.Metall Analy(in Chinese),2007,27(4):7–10. [6]张东,苏会东,高虹.火焰原子吸收法研究纳米钛酸锶钡粉体对铅的吸附性能[J].光谱学与光谱分析,2008,28(1):218–221.ZHANG Dong,SU Huidong,GAO Hong.Spectroscopy Spectral Analy(in Chinese),2008,28(1):218–221. [7]张东,苏会东,高虹,等.双硫腙包覆钛酸锶钡粉体对水中铅的吸附行为[J].化学学报,2007,65(22):2549–2554.ZHANG Dong,SU Huidong,GAO Hong,et al.Acta Chim Sin(in Chinese),2007,65(22):2549–2554. [8]张东,苏会东,高虹.双硫腙包覆钛酸锶钡粉体对水中镉的吸附性能[J].光谱学与光谱分析,2008,28(3):693–696.ZHANG Dong,SU Huidong,GAO Hong.Spectroscopy Spectral Analy(in Chinese),2008,28(3):693–696. [9]张东,张文杰,关欣,等.负载型纳米钛酸锶钡对水中Cd～(2+)吸附行为研究[J].光谱学与光谱分析,2009,29(3):824–828.ZHANG Dong,ZHANG Wenjie,GUAN Xin,et al.Spectroscopy Spectral Analy(in Chinese),2009,29(3):824–828. [10]王英泽,黄奔,吕娟,等.纳米技术在生物医学领域的研究现状[J].生物物理学报,2009,25(3):168–174.WANG Yingze,HUANG Ben,LüJuan,et al.Acta Biophysica Sinica(in Chinese),2009,25(3):168–174. [11]WANG Zheng,JIANG Shenglin,LI Guangxing,et al.Synthesis and characterization of Ba1·xSrxTiO3nanopowders by citric acid gel method[J].Ceram Int,2007,33:1105–1109. [12]吴瑾光.近代傅里叶变换红外光谱技术及应用(下卷)[M].北京:科学技术文献出版社,1994:193–212,256–323.WU Jinguang.FTIR Technology and Applications in Recent Years(Latter volume,in Chinese).Beijing:Science and Technology Litera-ture Press,1994:193–212,256–323. [13]赵振国,金明钟.氨基酸在固/水界面的吸附作用[J].离子交换与吸附,2001,17(5):289–295.ZHAO Zhenguo,JIN Mingzhong.Ion Exchange Adsorption(in Chi-nese),2001,17(5):289–295. [14]李明利,徐明霞,梁辉.Ba0.6Sr0.4TiO3料浆的稳定性研究[J].武汉理工大学学报,2007,29(10):124–127.LI Mingli,XU Mingxia,LIANG Hui.J Wuhan Univ Technol(in Chi-nese),2007,29(10):124–127. [15]张东,宋恩军,张丽丽,等.经丁二酮肟修饰的改性皂土对废水中镍的吸附[J].中国环境科学,2009,29(7):713–717.ZHANG dong,SONG Enjun,ZHANG Lili,et al.China Environm Sci(in Chinese),2009,29(7):713–717. [16]邵爽,邱瑾.金属离子对齐多夫定与牛血清白蛋白结合作用的影响[J].物理化学学报,2009,25(7):1342–1346.SHAO Shuang,QIU Jin.Acta Physico-Chimica Sinica(in Chinese),2009,25(7):1342–1346. [17]甘宏宇,商振华,刘学良,等.批量法研究牛γ-球蛋白在尼龙亲和膜上的等温吸附行为[J].分析化学,2001,29(6):637–641.GAN Hongyu,SANG Zhenhua,LIU Xueliang,et al.Chin J Analy Chem(in Chinese),2001,29(6):637–641.
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