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ProductionofFumaricAcidfromRawStarchyMaterialsbyRhizopusoryzaewithSSF

Keywords: fumaricacid,starchy,Rhizopusoryzae,carbon/nitrogenratio,simultaneoussaccharificationandfermentation

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Abstract:

Toreducethemanufacturingcostoffumaricacidproductionbyfermentation,fourkindsofrawstarchymaterialswereinvestigated,cassavapowder,cornpowder,degermedcornpowderandstarch.Bycomparingthenitrogencontentsinthem,carbon/nitrogenratioofrawmaterialswasthekeyfactoraffectingthefumaricacidproductionbyRhizopusoryzae.Basedonthispoint,degermedcornpowderwasselectedasthebestrawstarchymaterialforfumaricacidproduction.Inaddition,thesimultaneoussaccharificationandfermentation(SSF)processforfumaricacidproductionwasoptimized.Withdegermedcornpowderassubstrateat100g/Ltotalsugarconcentration,thefumaricacidproduction(35.51g/L),yield(0.355g/g)andproductivity(0.493[g/(L×h)])wereobtainedviaSSFprocessina7-Lfermentor.

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