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过程工程学报 2003
ProductionofCitricAcidfromApplePomaceEnzymolyzedbyCellulaseKeywords: citricacid,applepomace,cellulase,fermentation Abstract: CellulasecanevidentlyincreasethecontentofglucoseandhasasignificanteffectontheproductionofcitricacidfromapplepomacebyAspergillusniger.Basedonexperiments,acellulolyticenzymenamedcellulaseA6wasfoundabletoproduceabout170gglucosefrom1kgdriedapplepomaceafter12hreaction,withcellulaseconcentrationof20U/ginthemediumat50℃,naturalpHwithoutpretreatmentofalkali.Usingthetreatedapplepomaceasaliquidstatesubstrate,Aspergillusniger-Cselectedoutwasabletoproduceabout256gcitricacidfrom1kgdriedapplepomaceat35℃in3dor30℃in5dwithflaskrotationspeedof210r/min,andtheconversionofcitricacidcouldreach80%basedontheamountofsugarconsumed.
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