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环境化学  2015 

固定化多酚氧化酶对水中苯酚和氯酚的去除

Keywords: 多酚氧化酶,壳聚糖,蒙脱土,酚类化合物

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Abstract:

以壳聚糖/蒙脱土插层复合物为载体,吸附法制备固定化多酚氧化酶,并以此催化氧化去除水中的苯酚、4-氯苯酚和2,4-二氯苯酚.考察了固定化多酚氧化酶的制备条件,对酚类化合物的催化氧化条件、动力学特性以及固定化酶的重复使用性能.结果表明,固定化多酚氧化酶的最佳制备条件为pH5.0,酶与载体质量比20mg·g-1,固定化6h,所得固定化酶的载酶量为12.12mg·g-1,每克单位载体酶活为12.76×103U·g-1.固定化多酚氧化酶对酚类化合物的最佳去除条件为苯酚溶液pH7.0,温度30℃;4-氯苯酚溶液pH5.0,温度20℃;2,4-二氯苯酚溶液pH5.0,温度30℃.在最佳反应条件下,酶与底物质量比为20mg·mg-1时,固定化多酚氧化酶对苯酚、4-氯苯酚和2,4-二氯苯酚溶液去除率分别为63.6%、85.8%和87.8%.苯酚、4-氯苯酚和2,4-二氯苯酚的米氏常数Km值依次减小,最大反应速率Vmax依次增大,表明固定化酶对2,4-二氯苯酚的亲和力最强,催化速度最快.固定化酶循环使用6次(24h)后对苯酚、4-氯苯酚和2,4-二氯苯酚的去除率分别为15.7%、24.2%和27.8%.

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