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环境化学  2015 

槐糖脂生物表面活性剂的结构特征及理化性质初探

Keywords: 槐糖脂,发酵,高效液相色谱-电喷雾飞行时间质谱,表面张力,乳化性能

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Abstract:

通过摇瓶培养实验,考察了假丝酵母菌(Candidabombicola)ATCC22214以葡萄糖和油酸双底物为碳源,采用一次加料培养和分批补料培养方式进行发酵制备槐糖脂的过程,并利用高效液相色谱/电喷雾飞行时间质谱联用仪确定了所产槐糖脂的结构,初步研究了其主要的理化性质.结果表明,一次加料培养中,该菌株生长稳定期的中期即120h时槐糖脂产率最高,达45g·L-1,分批补料培养方式中,196h时糖脂产率达到101g·L-1.所产糖脂由7种乙酰基取代的含有17-羟基十八烯酸或十八烷酸的内酯型和酸型槐糖脂同系物构成,其中,保留时间为45.46min的17-L--十八烯酸-1',4″-内酯-6',6″二乙酸酯和保留时间48.35min的17-L--十八烷酸-1',4″-内酯-6',6″二乙酸酯比例较高,分别达27.38%和21.53%.该生物表面活性剂具有良好的表面活性和乳化能力,临界胶束浓度较低,为36.5mg·L-1.

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