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环境化学  2015 

丙酯草醚降解菌CY的分离、鉴定及其降解特性

Keywords: 丙酯草醚,芽孢杆菌属,16SrRNA,生物降解,降解特性

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Abstract:

采用富集培养的方法,从施用过丙酯草醚除草剂的油菜田中分离筛选出5株以丙酯草醚(ZJ0273)为唯一生长碳源和能源的菌株,选其中降解率高的菌株CY进行研究.根据其生理生化和16SrRNA基因序列相似性分析,鉴定其为芽孢杆菌属(Bacillussp.).根据对CY的初步研究和简单优化,菌株CY在30d内对50mg·L-1ZJ0273的降解率达到55.1%,该菌株降解ZJ0273的最适初始pH值为8.0,最适温度为35℃.菌株CY对ZJ0273的降解效果与初始接种量在一定范围内呈正相关,当初始浓度>400mg·L-1时,ZJ0273降解率偏低.

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