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环境化学  2015 

低剂量三氯生和三氯卡班对嗜热四膜虫的毒性研究

Keywords: 三氯生,三氯卡班,嗜热四膜虫,生态毒性,环境相关浓度

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Abstract:

研究了环境相关浓度水平(μg·L-1)的三氯生(TCS)和三氯卡班(TCC)暴露对嗜热四膜虫的生长抑制效应,并尝试探索这两种新生污染物对四膜虫细胞活性的影响.结果表明,μg·L-1水平的TCS和TCC对四膜虫的生长存在明显抑制作用,24h-EC50分别为141μg·L-1、728μg·L-1;最低无效应浓度(NOEC)分别为2μg·L-1、30μg·L-1;最低效应浓度(LOEC)分别为4μg·L-1、61μg·L-1.其中,TCC在1—10μg·L-1水平表现出促进作用,可能是hormesis效应的体现.另外,在TCS和TCC浓度达到1000μg·L-1时均对四膜虫细胞膜产生明显损伤;TCS和TCC也影响其溶酶体活性,1μg·L-1暴露2h时,荧光值(RFU)百分比分别降低至对照样品的88.62%、95.75%.本研究结果有助于进一步从亚细胞和分子水平认识环境污染水平TCS和TCC的生态毒性,也为这两种新生污染物在环境中的生态风险评价提供重要参考依据.

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