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环境化学  2015 

11种取代酚类内分泌干扰活性的初步筛选与评价

Keywords: 内分泌干扰物,,MTT法

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Abstract:

通过聚蔗糖泛影葡胺密度梯度离心法,从鲫鱼(Carassiusauratus)外周血中分离出淋巴细胞,体外培养24h,用MTT法测试双酚类、联苯酚类、二酚类、烷基单酚类和取代酚类等几类可疑环境内分泌干扰物对淋巴细胞增殖的影响.结果表明,双酚类、联苯酚类和烷基单酚类物质对鲫鱼淋巴细胞的增殖具有低浓度促进,高浓度抑制的作用.二酚类物质对鲫鱼淋巴细胞的增殖具有促进作用.取代酚类对鲫鱼淋巴细胞的增殖无明显作用.该结果表明,此方法可以对环境内分泌干扰物进行初步筛选,同时发现环境内分泌干扰物对淋巴细胞增殖的效应与物质的化学结构具有一定相关性.

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