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环境化学  2015 

超高效液相色谱-三重四极杆串联质谱法测定蜂蜜中吡咯里西啶类生物碱

Keywords: 吡咯里西啶类生物碱,超高效液相色谱-三重四极杆串联质谱法,蜂蜜

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Abstract:

建立了蜂蜜中5种吡咯里西啶类生物碱的超高效液相色谱-串联质谱测定方法.蜂蜜样品用50%甲醇超声提取,强阳离子交换固相萃取柱净化富集,超高效液相色谱-三重四极杆串联质谱MRM模式检测,外标法定量.以WatersBEHC18(100mm×2.1mm,1.7μm)色谱柱分离,以含0.1%甲酸的乙腈和0.1%甲酸水溶液进行梯度洗脱.倒千里光碱、千里光宁、千里光菲啉和野百合碱在0.2-100.0μg·kg-1,克氏千里光宁在1.0-200.0μg·kg-1的浓度范围内各化合物线性关系良好,相关系数r均大于0.999,定量限在0.2-0.8μg·kg-1之间.在3个加标水平下,空白基质蜂蜜中的5种生物碱的平均回收率为82.0%-110.0%之间,相对标准偏差(RSD)小于12.5%.方法准确可靠,适合于蜂蜜中吡咯里西啶类生物碱的测定.

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