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环境化学  2015 

孔雀草对镉胁迫的响应及其积累与分布特征

Keywords: 孔雀草,,耐性,亚细胞分布,化学形态

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Abstract:

采用营养液培养法研究了不同浓度的Cd(0、0.01、0.05、0.1、0.2、0.3、0.4mmol·L-1)对孔雀草叶片光合色素和丙二醛含量以及Cd积累量、亚细胞与化学形态分布的影响.结果表明,随着营养液Cd浓度的增加,叶片光合色素含量呈先升后降趋势,丙二醛含量则呈线性递增趋势,高浓度Cd处理(≥0.1mmol·L-1)对孔雀草产生了显著的胁迫响应.Cd主要贮存于可溶组分中,根系中占50.91%-66.40%,叶片中占39.09%-60.52%;其次为细胞壁,细胞器中的镉比例较低.随着Cd处理浓度的增加,Cd在根系细胞壁中的贮存比例呈增加趋势.液胞区隔化和细胞壁固持是孔雀草应对Cd胁迫的重要耐性机制.根系中的Cd主要以乙醇提取态存在,占27.62%-70.46%,叶片中Cd主要以去离子水提取态和氯化钠提取态存在,两者合计占58.91%-71.09%.叶片中活性态Cd含量显著低于根系,显著降低了地上部Cd的积累,也显著降低了Cd对地上部的胁迫作用.

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