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环境化学  2015 

柱前衍生化-气相色谱/质谱法测定细胞中的对-正壬基酚

Keywords: 对-正壬基酚,衍生化,气相色谱/质谱,细胞,细胞质

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Abstract:

采用柱前衍生化-气相色谱/质谱法测定染毒细胞以及细胞质中对-正壬基酚(4-n-NP)的含量,并初步探讨了4-n-NP在细胞中的分布.在不同介质中加标回收率在76.5%—98.9%之间.利用本方法测定实际样品发现在浓度为10μmol·l-1和20μmol·l-1的4-n-NP溶液中暴露12h,105个细胞中4-n-NP的含量分别为21.0ng和43.0ng,而相应的细胞质中4-n-NP的含量分别为14.2ng和28.4ng,显示有67.6%和66.0%的4-n-NP分布在细胞质中.

References

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