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化工进展  2015 

ClostridiumsaccharobutylicumDSM13864细胞表面理化特性及固定化细胞产丁醇的性能

DOI: 10.16085/j.issn.1000-6613.2015.12.014, PP. 4214-4219

Keywords: 糖丁基梭菌,丁醇,固定化,细菌吸附有机溶剂,砖块

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Abstract:

糖丁基梭菌ClostridiumsaccharobutylicumDSM13864能利用多种糖类为底物发酵产丁醇。本文研究了该菌体细胞表面的理化特性,并以砖块作为细胞固定化材料进行丁醇发酵。采用细菌吸附有机溶剂(MATS)法证明糖丁基梭菌细胞表面有强烈的亲水性,并且等电点在pH值为3左右,这些特性有利于菌体与表面亲水多孔的砖块吸附。在60g/L葡萄糖发酵培养基中,以5~8目砖块作为固定化材料,流速为1.1L/min,发酵48h后,丁醇的浓度、得率和生产率分别达到11.02g/L、0.18g/g和0.23g/(L·h),相比悬浮细胞发酵分别提高了10.53%、5.88%和9.52%。结果表明砖块作为一种固定化材料可有效提高糖丁基梭菌的发酵产丁醇水平。

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