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化工进展  2015 

高效功能菌群RR的筛选及其群落分析

DOI: 10.16085/j.issn.1000-6613.2015.07.027

Keywords: 染料脱色,混合菌群,群落分析,变性梯度凝胶电泳

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Abstract:

利用梯度浓度压力驯化法,从厌氧反应器中筛选出对直接红28有具有良好脱色能力的混合菌群RR。在染料初始浓度为200mg/L,pH=7.0,温度为35℃条件下,经48h静止培养,染料脱色率可达96.16%。进一步对其培养条件如pH值、温度、盐度、初始染料浓度等进行了脱色研究,结果表明,在pH=7、温度为45℃,盐度为2mmol/L的情况下,功能菌群脱色效果达到最佳。为了进一步适应工程无机条件,筛选出以染料作为唯一氮源、碳源以及能量的功能菌群,遂将培养基中葡萄糖去掉,同样利用梯度浓度压力驯化法,筛选出混合菌群RM,并对其脱色性能及群落结构进行分析。混合菌群RM在染料初始浓度为50mg/L、温度为35℃、pH=7.0条件下,48h后其脱色效率为20.05%。利用变性梯度凝胶电泳(DGGE)法,对群落进行分析,混合菌群RR主要为伯克霍尔德氏菌属(Burkholderiasp.)、链球菌属(Streptococcus)和克雷伯氏菌属(Klebsiellasp.),菌群RM主要为伯克霍尔德氏菌属(Burkholderiasp.),可见伯克霍尔德氏菌属菌株(Burkholderiasp.)可以适应工程无机环境,并对直接红28存在一定的降解能力。

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