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化工进展  2015 

自污染染料中筛选的菌对颜料紫1的脱色

DOI: 10.16085/j.issn.1000-6613.2015.04.030

Keywords: 染料脱色,杂色曲霉菌,颜料紫1,响应面法,脱色机制

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Abstract:

为研究颜料紫1的脱色问题,从一盒长满菌苔的广告浓缩染料(主要显色原料颜料紫1)中筛选菌株,通过摇瓶培养、以及平板分离等获得了单一纯种菌株、并进一步对该菌株进行了18SrDNA测序分析,从而鉴定出该菌为杂色曲霉(Aspergillusversicolor).在此基础上,系统地研究了该菌株对颜料紫1的脱色条件.综合单因素实验和响应面法优化实验的结果表明该杂色曲霉菌对于颜料紫1脱色的最佳条件为碳源葡萄糖30g/L,颜料紫1起始含量为250mg/L,NaNO3含量为0.3g/L,pH值5.5,这时可获得最高的颜料紫1脱色率为84.64%.最后,通过傅里叶变换红外光谱仪(FTIR)分析,揭示了该杂色曲霉菌对颜料紫1的脱色机制主要为吸附脱色.

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