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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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侧链含咪唑取代基聚(L-天冬酰胺)高效基因载体研究
DOI: 10.3724/SP.J.1105.2011.11114, PP. 874-882
Keywords: 基因传递,聚阳离子,聚(L-天冬酰胺),咪唑,低毒
Abstract:
通过氨基引发聚丁二酰亚胺(PSI)开环反应,制备了系列侧链含氨乙基和咪唑丙基的聚(L-天冬酰胺)共聚物(P1~P5).该系列聚合物不仅具有极低的细胞毒性,而且随侧链中咪唑取代基含量的增加,聚合物在pH5~8范围内缓冲能力显著提高.通过凝胶电泳、粒径和电位分析等研究了聚合物与质粒DNA的相互作用.结果表明,所有聚合物均可以在较低N/P比有效结合DNA.在N/P比高于5时,形成粒径在100nm以下、表面电荷为+20mV左右的稳定复合物纳米粒子.聚合物介导荧光素酶基因(pGL-3)和增强型绿色荧光蛋白基因(pEGFP-C1)体外转染293T细胞和COS-7细胞的结果表明,侧链中引入咪唑取代基显著提高了聚合物的转染能力.通过优化侧链氨基/咪唑取代基比例,获得转染效率最高的聚合物P4.P4介导pGL-3和pEGFP-C1在细胞中的表达与聚乙烯亚胺(PEI)相当、甚至是PEI的2~3倍.
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