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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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应用光亲和分子进行PEG的光照修饰及蛋白质的光照偶联
DOI: 10.3724/SP.J.1105.2011.11080, PP. 1341-1348
Keywords: 光亲和标记,光敏感聚合物,蛋白质固定,PEG修饰
Abstract:
合成了一种光活性标记分子-对叠氮苯甲酸,将其偶联到具有双羟基的碳酸酯与乳酸的共聚物P(LA-co-DHP)上,获得了具有光反应活性的可生物降解共聚物P(LA-co-DAP),在光照条件下,可以将蛋白质方便快捷地共价偶联到P(LA-co-DHP)聚合物纤维上.在溶液中进行PEG与对叠氮苯甲酸的光照反应,通过核磁共振光谱检测,证明了对叠氮苯甲酸可以直接修饰到PEG侧链上,形成苯甲酸侧基PhCOOH.这是其它化学修饰方法很难做到的.实验还证明,对叠氮苯甲酸对PEG的光亲和修饰不但能在溶液中进行,而且能在PEG自组装膜的表面进行.
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