OALib Journal期刊
巯基化改性壳聚糖在电化学生物传感器制备中的应用
DOI: 10.3724/SP.J.1105.2013.12244
Keywords: 巯基壳聚糖 ,纳米金 ,修饰电极 ,循环伏安 ,细胞色素c ,过氧化氢
Abstract:
以壳聚糖、N-乙酰-L-半胱氨酸(NAC)为原料,以1-羟基苯并三唑(HOBt)和1-乙基-3-(3-二甲基胺丙基)碳化二亚胺盐酸盐(EDAC)为缩合剂,合成功能化壳聚糖衍生物巯基壳聚糖(CHS-NAC).用红外光谱(FTIR)、核磁共振(1H-NMR)及X射线衍射(XRD)对其结构进行表征,用Ellman’s试剂通过标准曲线法测得巯基含量.利用CHS-NAC的黏附性,通过层层吸附的方法将CHS-NAC、纳米金及细胞色素c分别修饰到玻碳电极(GC)上,通过扫描电子显微镜(SEM)对修饰电极表面的形貌进行了观察,采用循环伏安和电化学阻抗研究了不同修饰膜电极的电化学行为,及扫描速率对细胞色素c修饰电极的影响,并开展了对过氧化氢的电催化分析.实验结果表明,CHS-NAC能高效地将纳米金及细胞色素c固定在电极表面,并能有效发挥纳米金辅助转移电子及细胞色素c对过氧化氢催化的能力.
References
[1] 5 Aranaz I,Harris R,Heras A.Current Organic Chemistry,2010,14(1):308~330 [2] 6 Shao M L,Bai H J,Gou H L,Xu J J,Chen H Y.Langmuir,2009,25(5):3089~3095 [3] 7 Tang J,Tang D P,Li Q F,Su B L,Qiu B,Chen G N.Analytica Chimica Acta,2011,697(1-2):16~22 [4] 9 Xu S J,Liu Y,Wang T H,Li J H.Anal Chem,2011,83(10):3817~3823 [5] 15 Pozzo A D,VaniniL F M,Guerrini M,Benedittis A,Muzzarelli R A A.Carbohydrate Polym,2000,42(1):201~206 [6] 16 Wang Xin(王鑫),Wu Zhongming(毋中明),Zhang Xinge(张新歌),Zheng Chao(郑超),Wang Zhen(王镇),Li Zhaoxing(李朝兴).Chem J Chinese U(高等学校化学学报),2008,29(4):851~857 [7] 17 Lv Lin(吕霖),Zhou Jianping(周建平),Zhang Yong(张勇).Chinese New Drug Journal(中国新药杂志),2007,16(16):1250~1254 [8] 18 Zhang Yan(张雁),Kang Tanfang(康天放),Lu Liping(鲁理平),Liu Tongsheng(刘桐珅),Cheng Shuiyuan(程水源).Environmental Science (环境科学),2011,32(4):1127~1132 [9] 19 Kang X B,Pang G C,Liang X Y,Wang M,Liu J,Zhu W M.Electrochimica Acta,2012,62(1):327~334 [10] 20 Zheng Haixia(郑海霞),Huang Boneng(黄博能),Hu Junman(胡君曼),Gong Yan(龚).Acta Scientiarum Naturalium Universitatis Pekinensis(北京大学学报(自然科学版)),2011,47(5):777~782 [11] 23 Dung P M,Milas M,Rinaudo M,Desbriers J.Carbohydr Polym,1994,24(3):209~214 [12] 24 Sankararamakrishnan N,Sanghi R.Carbohydr Polym,2006,66(2):160~167 [13] 25 Sun Gangzheng(孙刚正).Preparation,Characteristics Evaluation of Oleoyl-arboxymethyl Chitosan as a Coagulation Agent(羧甲基油酞壳聚糖的制备、性质及其对含油废水絮凝机理的研究).Doctoral Dissertation of China Ocean University(中国海洋大学博士学位论文).2010 [14] 1 Zhang Ya(张亚),Zheng Jianbin(郑建斌).Acta Chim Sinica(化学学报),2011,69(16):1903~1908 [15] 2 Xu Jiming(徐继明),Li Wei(李伟),Yin Qifan(尹起范),Zhu Yulan(朱玉兰).Chemical Research and Application(化学研究与应用),2007,19(4):370~373 [16] 3 Bai Linshan(白林山),Han Rui(韩睿),Lv Dandan(吕丹丹).Journal of Anhui University of Technology (安徽工业大学学报(自然科学版)), 2011,28(3):375~379 [17] 4 Qu Jianying(屈建莹),Chen Wenjing(陈文静),Wang Yu(王钰),Zhu Lili(朱莉莉).Journal of Analytical Science(分析科学学报),2011,27(3):363~366 [18] 8 Lai G S,Yan F,Wu J,Leng C,Ju H X.Anal Chem,2011,83(7):2726~2732 [19] 10 Hu Yintao(胡印滔),Li Meihua(李梅华),Li Mingchun(李明春).Chemical Industry and Engineering Progress (化工进展),2011,30(10):2228~2234 [20] 11 Zhu Jingtuo(祝敬妥),Zhang Hui(张卉),Xu Jingjuan(徐静娟),Chen Hongyuan(陈洪渊).Journal of Analytical Science(分析科学学报),2009,25(1):1~5 [21] 12 Liang Kezhong(梁克中).Chemical Research and Application(化学研究与应用),2010,22(12):1520~1525 [22] 13 Liu Y,Li Y,Liu S,Li J,Yao S.Biomater,2004,25(26):5725~5733 [23] 14 Huang H,Yang X.Colloids Surf A:Physicochem Eng Aspects,2003,226(1-3):77~86 [24] 21 John Lin Z P,Ji W H,Daksha D K,Shum L Y.J Pharm Biomed Anal,2003,33(1):101~108 [25] 22 Zheng Yu(郑瑜),Lin Xiangqin(林翔钦).Chinese Journal of Analytical Chemistry(分析化学),2008,36(5):604~608 [26] 26 Chen S H,Yuan R,Chai Y Q.Biosens Bioelectron,2007,22(7):1268~1274 [27] 27 Luo Qimei(罗启枚),Wang Huixian(王辉宪),Xu Xinyu(徐新宇),Li Yantao(李彦涛),Huang Jun(黄俊),Liu Dengyou(刘登友).Journal of Instrumental Analysis(分析测试学报),2011,30(7):780~783
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