|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
利用聚合物自组装实现绿色荧光蛋白生色团的荧光增强
DOI: 10.3724/SP.J.1105.2013.12418, PP. 660-667
Keywords: 绿色荧光蛋白,荧光生色团,两亲性嵌段聚合物,自组装,成像
Abstract:
根据绿色荧光蛋白的发光原理,采用聚乙二醇与聚甲基丙烯酸甲酯的两亲性两嵌段聚合物通过自组装包覆生色团的方式,模拟了绿色荧光蛋白发光,考察了组装行为对光学性能的影响,并将其用于细胞成像.通过核磁共振、高分辨质谱、傅里叶变换红外光谱、凝胶渗透色谱、紫外-可见吸收光谱及荧光光谱等表征了生色团分子和聚合物的结构及性能.生色团紫外最大吸收在371nm,荧光最大发射峰在428nm.聚合物和生色团进行组装后,其紫外吸收消失,而最大荧光发射峰强度大大增强,且发生了约70nm的红移,这是因为组装使得生色团的自由旋转受到了限制,且生色团共平面性增加.动态光散射(DLS)和透射电镜(TEM)证明了纳米粒子的结构和尺寸.由于尺寸适合且具有较好的荧光性能,纳米粒子成功应用于细胞成像.这种绿色荧光蛋白生色团的简单自组装方式在生物成像领域具有良好应用前景.
| [1] | 3 Brejc K B,Sixma T K,Kitts P A,Kain S R,Tsien R Y,Orm？ M,Remington S J.Proc Natl Acad Sci,1997,94:2306~2311
|
| [2] | 4 Tsien R Y,Annu Rev Biochem,1998,67:509~544
|
| [3] | 6 Baird G S,Zacharias D A,Tsien R Y.Proc Natl Acad Sci,2000,97:11984~11989
|
| [4] | 7 Labas Y A,Gurskaya N G,Yanushevich Y G,Fradkov A F,Lukyanov K A,Lukyanov S A,Matz M V.Proc Natl Acad Sci,2002,99:4256~4261
|
| [5] | 8 Zimmer M.Chem Rev,2002,102:759~781
|
| [6] | 13 Niwa H,Inouye S,Hirano T,Matsuno T,Kojima S,Kubota M,Ohashi M,Tsuji F I.Proc Natl Acad Sci,1996,93:13617~13622
|
| [7] | 14 Dong J,Solntsev K M,Tolbert L M.J Am Chem Soc,2006,128:12038~12039
|
| [8] | 15 Dong J,Solntsev K M,Poizat O,Tolbert L M.J Am Chem Soc,2007,129:10084~10085
|
| [9] | 16 Sakamoto S,Kudo K,J Am Chem Soc,2008,130:4089~4096
|
| [10] | 18 Chen K Y,Cheng Y M,Lai C H,Hsu C C,Ho M L,Lee G H,Chou P T.J Am Chem Soc,2007,129:4534~4535
|
| [11] | 19 Baldridge A,Samanta S R,Jayaraj N,Ramamurthy V,Tolbert L M.J Am Chem Soc,2010,132:1498~1499
|
| [12] | 24 Tolbert L M,Baldridge A,Kowalik J,Solntsev K M.Acc Chem Res,2012,2:171~181
|
| [13] | 25 Baldridge A,Solntsev K M,Song C,Tanioka T,Kowalik J,Hardcastle K,Tolbert L M.Chem Commun,2010,46:5686~5688
|
| [14] | 26 Baldridge A,Kowalik J,Tolbert L M.Synthesis,2010,14:2424~2436
|
| [15] | 1 Prasher D, McCann R O,Cormier M J.Biochem Biophys Res Commun,1985,126:1259~1268
|
| [16] | 2 Cubitt A B,Heim R,Adams S R,Boyd A E,Gross L A,Tsien R Y.Trends Biochem Sci,1995,20:448~455
|
| [17] | 5 Wall M A,Socolich M,Ranganathan R.Nature Struct Biol,2000,7:1133~1138
|
| [18] | 9 Miyawaki A.Develop Cell,2003,4:295~305
|
| [19] | 10 Meech S R.Chem Soc Rev,2009,38:2922~2934
|
| [20] | 11 Craggs T D.Chem Soc Rev,2009,38:2865~2875
|
| [21] | 12 Heim R,Prasher D C,Tsien R Y.Proc Natl Acad Sci,1994,91:12501~12504
|
| [22] | 17 Wu L X,Burgess K.J Am Chem Soc,2008,130:4089~4096
|
| [23] | 20 Baldridge A,Feng S,Chang Y T,Tolbert L M.ACS Comb Sci,2011,13:214~217
|
| [24] | 21 Sakamoto S,Kudo K.J Am Chem Soc,2008,130:9574~9582
|
| [25] | 22 Deng H P,Zhu Qi,Wang D L,Tu C L,Zhu B S,Zhu X Y.Polym Chem,2012,3:1975~1977
|
| [26] | 23 Deng Hongping(邓洪平),Zhu Qi(朱琦),Wang Dali(王大力),Zhu Bangshang(朱邦尚),Zhu Xinyuan(朱新远).Acta Polymerica Sinica(高分子学报),2012,(10):91~97
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
