|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
末端功能化的树枝状聚合物载体与质粒组装的热力学和动力学
DOI: 10.3724/SP.J.1105.2013.12359, PP. 1025-1032
Keywords: 聚酰胺-胺树枝状聚合物,基因传递,质粒组装,光散射,恒温滴定量热,转染
Abstract:
通过二代和四代聚酰胺-胺树枝状聚合物(G2和G4表示)末端分别修饰苯丙氨酸和色氨酸合成了多种新型聚合物载体,进而考察了其与质粒的组装行为.研究结果显示,Phe-G4和Trp-G4与质粒组装为结构致密的纳米粒子,其中Phe-G4与质粒形成的纳米粒子更加紧密.这一过程是由聚合物载体与质粒间的静电相互作用和结构重排共同驱动的.此外,末端修饰方法显著降低了载体的细胞毒性.细胞转染结果表明,Phe-G4和Trp-G4成功介导质粒pEGFP-CL在COS-7细胞中表达,Phe-G4/pEGFP-CL的转染效率提高到作为阳性对照的脂质体组的4倍以上.因此,Phe-G4载体是一种有应用潜力的新型基因传递系统.
| [1] | 1 Ainalem M L, Nylander T.Soft Matter,2011,7(10):4577~4594
|
| [2] | 2 Huang Shiwen(黄世文),Zhuo Renxi(卓仁禧).Chin Sci Bull(科学通报),2003,48(5):405~409
|
| [3] | 3 Xu C H,Sui M H,Tang J B,Shen Y Q.Chinese J Polym Sci,2011,29(3):274~287
|
| [4] | 4 Park T G,Jeong J H,Kim S W.Adv Drug Deliv Rev,2006,58(4):467~486
|
| [5] | 5 Grigsby C L,Leong K W.J Roy Soc Int,2010,7:S67~S82
|
| [6] | 6 Sun X,Zhang N.Mini-Rev Med Chem,2010,10(2):108~125
|
| [7] | 7 Kim T,Seo H J,Choi J S,Jang H S,Baek J,Kim K,Park J S.Biomacromolecules,2004,5(6):2487~2492
|
| [8] | 8 Wang C H,Hsiue G H.Bioconjug Chem,2005,16(2):391~396
|
| [9] | 9 Xue Yanan(薛亚楠),Zhang Min(张敏),Liu Lei(刘磊),Li Wenxin(李文鑫),Huang Shiwen(黄世文).Acta Polymerica Sina(高分子学报),2011,(8):874~882
|
| [10] | 10 Ferber D.Science,2001,294(5547):1638~1642
|
| [11] | 11 Dame R T.Mol Microbiol,2005,56(4):858~870
|
| [12] | 12 Aris A,Villaverde A.Trends Biotechnol,2004,22(7):371~377
|
| [13] | 13 Yoshihara C,Shew C Y,Ito T,Koyama Y.Biophys J,2010,98(7):1257~1266
|
| [14] | 14 Chow Hak-Fun(周克勋).YouJi HuaXue(有机化学),1997,17(1):72~75
|
| [15] | 19 Huang R Q,Qu Y H,Ke W L,Zhu J H,Pei Y Y,Jiang C.Faseb J,2007,21(4):1117~1125
|
| [16] | 20 Hobson L J,Feast W J.Polymer,1999,40(5):1279~1297
|
| [17] | 23 Braun C S,Vetro J A,Tomalia D A,Koe G S,Koe J G,Middaugh C R.Journal of Pharmaceutical Sciences,2005,94(2):423~436
|
| [18] | 24 Lai E,van Zanten J H.Biophys J,2001,80(2):864~873
|
| [19] | 25 Jensen L B,Pavan G M,Kasimova M R,Rutherford S,Danani A,Nielsen H M,Foged C.Int J Pharm,2011,416(2):410~418
|
| [20] | 15 Svenson S,Tomalia D A.Adv Drug Deliv Rev,2005,57(15):2106~2129
|
| [21] | 16 Eichman J D,Bielinska A U,Kukowska-Latallo J F,Baker J R,Jr.Pharm Sci Technolo Today,2000,3(7):232~245
|
| [22] | 17 Choi J S,Nam K,Park J,Kim J B,Lee J K.J Control Release,2004,99(3):445~456
|
| [23] | 18 Sambrook J,Russell D W.Molecular Cloning:A Laboratory Manual.New York:CSHL Press,2001.387~395
|
| [24] | 21 Rungsardthong U,Ehtezazi T,Bailey L,Armes S P,Garnett M C,Stolnik S.Biomacromolecules,2003,4(3):683~690
|
| [25] | 22 Tan J F,Too H P,Hatton T A,Tam K C.Langmuir,2006,22(8):3744~3750
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
