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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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载血红蛋白纳米胶束的制备及蛋白封装行为研究
DOI: 10.11777/j.issn1000-3304.2015.14352, PP. 535-541
Keywords: ATRP,纳米胶束,咪唑,血红蛋白,人工氧载体
Abstract:
通过原子转移自由基聚合(ATRP)制备了两亲性嵌段共聚物聚乙二醇-聚甲基丙烯酸缩水甘油酯PEG-b-P(GMA)45,利用1-(3-氨基丙基)咪唑(API)与GMA上的环氧基发生开环反应,合成了聚乙二醇-聚(咪唑丙基-1-胺-甲基丙烯酸羟丙酯)PEG-P(GMA-API)45.研究表明,该聚合物可以直接在水中自组装形成均匀的球形纳米胶束,粒径约为40nm.由于咪唑基的pH响应性,胶束表面在pH从4增大到9的范围内均带正电荷,pH7.4时共聚物可以与血红蛋白产生较强的静电作用和氢键作用,达到包载血红蛋白的目的.同时还研究了聚合物对不同比例血红蛋白的包封率,当聚合物与血红蛋白的质量比为11时包封率最高,为32.4wt%.UV-Vis显示包封后的血红蛋白在不同气体结合状态的特征吸收峰与自由血红蛋白差别不大,表明载血红蛋白纳米胶束具有良好的气体运输能力,PEG-P(GMA-API)45纳米胶束将有可能用于新型的人工氧载体.
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