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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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复合胶束辅助蛋白酶复性的研究
DOI: 10.11777/j.issn1000-3304.2014.14096, PP. 1561-1567
Keywords: 温敏性聚合物,复合胶束,蛋白质折叠,人工伴侣,疏水作用
Abstract:
提出了一种使用复合胶束模拟分子伴侣来辅助蛋白质折叠的方法.制备了3种由不同比例的嵌段共聚物聚乳酸-b-聚(N-异丙基丙烯酰胺)(PLA-b-PNIPAM)与聚乳酸-b-聚乙二醇(PLA-b-PEG)组成的温敏性的复合胶束(mixed-shellpolymericmicelles,MSPMs),通过动态光散射考察了复合胶束在37℃以及在变性剂存在下的稳定性.以碳酸酐酶(CAB)和脂肪酶(Lipase)作为模型蛋白,研究了MSPM辅助蛋白质折叠的能力.结果表明,在高浓度变性剂存在下,PNIPAM仍具有温敏性,并且摩尔比为11的PLA-b-PNIPAM和PLA-b-PEG制备的MSPM具有非常好的稳定性.不同比例的MSPM对于CAB和Lipase都具有辅助复性效果,远远高于没有MSPM时的自发复性.其中,摩尔比为11的MSPM使CAB的复性率高达92.1%,Lipase的复性率也达到50%.
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