|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
聚乙烯亚胺介导siRNA和顺铂协同抗肿瘤治疗
DOI: 10.11777/j.issn1000-3304.2015.14259, PP. 127-132
Keywords: 聚乙烯亚胺,Survivin基因,顺铂,协同作用,抗肿瘤
Abstract:
用聚乙烯亚胺(PEI)为载体,介导siRNA(siSurvivin)沉默肿瘤细胞抗凋亡基因survivin,并与抗癌药物(顺铂)进行协同抗肿瘤治疗.凝胶阻滞电泳实验显示,PEI能够对siRNA进行有效复合,在PEI/siRNA质量比为0.4时实现完全阻滞.细胞耐药性实验证明了耐顺铂细胞(A549DDP细胞)的survivin基因过度表达且耐顺铂能力是顺铂敏感细胞(A549细胞)的8倍.RT-PCR实验验证了PEI担载siSurvivin后对survivin基因实现了有效沉默,与顺铂药物共同作用后不影响基因沉默效果.细胞凋亡实验验证了基因与药物协同作用后细胞的凋亡率达到60.9%,而单独药物或PEI/siSurvivin复合物分别作用后的细胞凋亡率仅分别为30.2%和19.8%.细胞增殖实验进一步验证了PEI介导siSurvivin与顺铂联合治疗能够实现有效地协同抗肿瘤效果.
| [1] | 1 Verma I M, Somia N.Nature, 1997, 389:239~242
|
| [2] | 2 Fu Huili(付慧莉), Cheng Sixue(程巳雪), Zhuo Renxi(卓仁喜).Acta Polymerica Sinica(高分子学报), 2009, (1):97~103
|
| [3] | 3 Ghosh S S, Takahashi M, Thummala N R, Parashar B, Chowdhury N R, Chowdhury J R.J Hepatol, 2000, 32:238~252
|
| [4] | 4 Caplen N J, Parrish S, Imani F, Fire A, Morgan R A.Proceed Nat Acad Sci USA, 2001, 98:9742~9747
|
| [5] | 5 Chen J, Tian H Y, Dong X, Guo Z P, Jiao Z X, Li F F, Kano A, Maruyama A, Chen X S.Macromol Biosci, 2013, 13:1438~1446
|
| [6] | 6 Qi R G, Wu S H, Wang Y, Chen J, Xie Z G, Huang Y B, Jing X B.Chinese J Polym Sci, 2013, 31(6):912~923
|
| [7] | 7 Zhang Wei(张维), Liu Xiangli(刘相丽), Zhang Meng(张猛), Shi Qqinghong(史清洪).Acta Polymerica Sinica(高分子学报), 2013, (8):1025~1032
|
| [8] | 8 Dong Xuan(董璇), Tian Huayu(田华雨), Chen Jie(陈杰), Xia Jialiang(夏加亮), Chen Xuesi(陈学思), Wei Yen(危岩).Acta Polymerica Sinica(高分子学报), 2011, (9):1086~1091
|
| [9] | 9 Yuan Renxu(苑仁旭), Zuo Yufang(左瑜芳), Xiao Zhongpeng(肖中鹏), Huang Wenlin(黄文林), Shuai Xintao(帅心涛).Acta Polymerica Sinica(高分子学报), 2009, (2):104~110
|
| [10] | 10 Lane D.Nat Biotechnol, 2006, 24:163~164
|
| [11] | 11 Spankuch B, Heim S, Kurunci-Csacsko E, Lindenau C, Yuan J P, Kaufmann M, Strebhardt K.Cancer Res, 2006, 66:5836~5846
|
| [12] | 12 Song W T, Tang Z H, Li M Q, Lv S X, Sun H, Deng M X, Liu H Y, Chen X S.Acta Biomater, 2014, 10:1392~1402
|
| [13] | 13 Song W T, Tang Z H, Zhang D W, Zhang Y, Yu H Y, Li M Q, Lv S X, Sun H, Deng M X, Chen X S.Biomaterials, 2014, 35:3005~3014
|
| [14] | 14 Tian H Y, Chen J, Chen X S.Small, 2013, 9:2034~2044
|
| [15] | 15 Tian H Y, Tang Z H, Zhuang X L, Chen X S, Jing X B.Prog Polym Sci, 2012, 37:237~280
|
| [16] | 16 Zhu C H, Jung S, Luo S B, Meng F H, Zhu X L, Park T G, Zhong Z Y.Biomaterials, 2010, 31:2408~2416
|
| [17] | 17 Sun T M, Du J Z, Yao Y D, Mao C Q, Dou S, Huang S Y, Zhang P Z, Leong K W, Song E W, Wang J.Acs Nano, 2011, 5:1483~1494
|
| [18] | 18 Wang Y H, Zheng M, Meng F H, Zhang J, Peng R, Zhong Z Y.Biomacromolecules, 2011, 12:1032~1040
|
| [19] | 19 Song W T, Li M Q, Tang Z H, Li Q S, Yang Y, Liu H Y, Duan T C, Hong H, Chen X S.Macromol Biosci, 2012, 12:1514~1523
|
| [20] | 20 Trabulo S, Cardoso A M, Santos-Ferreira T, Cardoso A L, Simoes S, De Lima M C P.Mol Pharm, 2011, 8:1120~1131
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
