|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
复合转化生长因子-β1的磺化壳聚糖/聚赖氨酸纳米粒子的制备及其体外诱导干细胞分化性能
DOI: 10.3724/SP.J.1105.2013.12425, PP. 1177-1182
Keywords: 磺化壳聚糖,转化生长因子-β1,纳米粒子,骨髓间充质干细胞,分化
Abstract:
采用共沉淀法制备了复合转化生长因子-β1(TGF-β1)的磺化壳聚糖/聚赖氨酸纳米粒子(SCS/PLL/TGF-β1),研究了磺化壳聚糖的浓度对纳米粒子粒径和表面电位的影响.所得纳米微粒的粒径在240~290nm、表面电位在-0.4~-1.3mV之间可调.采用透射电子显微镜表征了磺化壳聚糖/聚赖氨酸(SCS/PLL)和SCS/PLL/TGF-β1纳米粒子的形貌.通过体外骨髓间充质干细胞(BMSCs)培养实验,分别考察了SCS/PLL/TGF-β1纳米粒子对BMSCs的增殖、氨基葡聚糖(GAGs)分泌和Ⅱ型胶原基因表达的影响.结果显示,相对于等量的SCS/PLL或同浓度的自由TGF-β1,SCS/PLL/TGF-β1纳米粒子可有效保护TGF-β1的活性,促进BMSCs向软骨细胞分化,可望应用于软骨修复材料等领域的研究.
| [1] | 2 Cheung H Y,Lau K T,Lu T P,Hui D.Composites:Part B,2006,38(2007):291~300
|
| [2] | 5 Wang W,Li D,Wang M,Li Y,Gao C.Chinese J Polym Sci,2011,29(2):233~240
|
| [3] | 12 Chatterjee K,Gibson S L,Wallace W E,Parekh S H,Lee Y J,Cicerone M T,Young M F,SimonJr C G.Biomaterials,2010,31(19):5051~5062
|
| [4] | 13 Puceat M.Cardiovasc Res,2007,74(2):256~261
|
| [5] | 14 Attur M G,Palmer G D,Al-Mussawir H E,Dave M,Teixeira C C,Rifkin D B,Appleton C T,Beier F,Abramson S B.FASEB J,2009,23(1):79~89
|
| [6] | 20 Zhao B,Katagiri T,Toyoda H,Takada T,Yanai T,Fukuda T,Chung U,Koike T,Takaoka K,Kamijo R.J Biol Chem,2006,281(32):23246~23253
|
| [7] | 21 Bian L,Zhai D Y,Tous E,Rai R,Mauck R L,Burdick J A.Biomaterials,2011,32(27):6425~6434
|
| [8] | 22 Go D P,Gras S L,Mitra D,Nguyen T H,Stevens G W,Cooper-White J J,Connor A J.Biomacromolecules,2011,12(5):1494~1503
|
| [9] | 23 Zhang Hanwei(张汉威),Cai Qing(蔡晴),Bei Jianzhong(贝建中),Wang Shenguo(王身国).Acta Polymeric Sinica(高分子学报),2004,(3):454~457
|
| [10] | 24 Peschel D,Zhang K,Fischer S,Groth T.Acta Biomater,2012,8(1):183~193
|
| [11] | 25 Huang Aibin(黄爱宾),Guo Rui(郭瑞),Xu Shaojun(徐少骏),Ma Lie(马列),Gao Changyou(高长有).Acta Polymeric Sinica(高分子学报),2009,(2):111~117
|
| [12] | 27 Wu Xingjie(吴兴杰),Ma Lie(马列),Gao Changyou(高长有).Acta Polymeric Sinica(高分子学报),2012,(4):418~426
|
| [13] | 28 Zhou H.Qian J,Wang J,Yao W,Liu C,Chen J,Cao X.Biomaterials,2009,30(9):1715~1724
|
| [14] | 29 Xing R,Liu S,Yu H,Guo Z,Li Z,Li P.Carbohydr Res,2004,339(15):2515~2519
|
| [15] | 1 Xu Rongyao(许荣耀), Zhang Shou(张寿).Journal of Clinical Rehabilitative Tissue Engineering Research(中国组织工程研究与临床康复),2011,15(41):7747~7750
|
| [16] | 3 Deplaine H,Lebourg M,Ripalda P,Vidaurre A,Sanz-Ramos P,Mora G,Prosper F,Ochoa I,Doblare M,Gomez J L,Izal-Azcarate I,Ferrer G G.J Biomed Mater Res B Appl Biomater,2013,101(1):173~186
|
| [17] | 4 Fu Huili(付慧莉),Cheng Sixue(程巳雪),Zhuo Reixi(卓仁禧).Acta Polymeric Sinica(高分子学报),2009,(2):97~103
|
| [18] | 6 Shi Xiaowen(施晓文),Li Xiaoxia(李晓霞),Du Yumin(杜予民).Acta Polymeric Sinica(高分子学报),2011,(1):1~11
|
| [19] | 7 Khan W S,Johnson D S,Hardingham T E.Knee,2010,7(6):369~374
|
| [20] | 8 Park J S,Na K,Woo D G,Yang H N,Park K H.Biomaterials,2009,30(27):4796~4805
|
| [21] | 9 Whitaker M J,Quirk R A,Howdle S M,Shakesheff K M.J Pharm Pharmacol,2001,53(11):1427~1437
|
| [22] | 10 Kopesky P W,Vanderploeg E J,Kisiday J D,Frisble D V,Sandy J D,Grodzinsky A J.Tissue Eng Part A,2011,17(1-2):83~92
|
| [23] | 11 Wang W,Li B,Li Y,Jiang Y,Ouyang H,Gao C.Biomaterials,2010,31(23):5953~5965
|
| [24] | 15 Kingsley D M.Genes Dev,1994,8(2):133~146
|
| [25] | 16 Blaney D E,Vitters E L,Berg W B,Kraan P M.Arthritis Res Ther,2006,8(3):65
|
| [26] | 17 Yang Guan(杨冠),Yang Xiao(杨晓).Hereditas(遗传),2008,30(8):953~959
|
| [27] | 18 Shen Y H,Shoichet M S,Radisic M.Acta Biomater,2008,4(3):477~489
|
| [28] | 19 Choi J S,Leong K W,Yoo H S.Biomaterials,2008,29(5):587~596
|
| [29] | 26 Wang H,Yuan L,Zhao T,Huang H,Chen H,Wu D.Chinese J Polym Sci,2012,30(6):893~899
|
| [30] | 30 Wang W,Li B,Yang J,Xin L,Li Y,Yin H,Qi Y,Jiang Y,Ouyang H,Gao C.Biomaterials,2010,31(34):8964~8973
|
| [31] | 31 Mauck R L,Yuan X,Tuan R S.Osteoarthritis Cartilage,2006,14(2):179~189
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
