OALib Journal期刊
L-肉碱分子表面印迹聚合物的制备及其识别性能研究
DOI: 10.3724/SP.J.1105.2014.13268
Keywords: 分子表面印迹 ,L-肉碱 ,甲基丙烯酸 ,手性识别 ,特异吸附
Abstract:
以L-肉碱（L-carnitine）作为印迹模板分子，甲基丙烯酸（MAA）为功能单体，乙二醇二甲基丙烯酸酯（EGDMA）为交联剂，采用硅胶表面分子印迹技术合成了L-肉碱分子印迹聚合物（MIP）和非印迹聚合物（NMIP）.通过紫外光谱研究了MAA与L-肉碱之间的结合作用.利用IR和SEM测试分别对产物进行了结构表征和表面形貌观察，说明分子印迹聚合物成功接枝到了硅胶表面.吸附动力学实验结果表明，MIP对L-肉碱有较好的识别性和吸附性；Scatchard分析表明该印迹聚合物中存在着一类等价的结合位点，最大表观结合量为71.00μmol/g，离解常数Kd=2.76×10-4mol/L；选择性吸附实验结果表明，MIP对L-肉碱的吸附结合量高于其D型异构体和其他类似物；拆分实验结果表明，MIP对DL-肉碱的拆分有一定作用.
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