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人脐带与骨髓来源间充质干细胞miRNA的差异表达

Keywords: 脐带间充值干细胞(UMSCs),骨髓间充值干细胞(BMSCs),miRNA,基因芯片,实时荧光定量PCR

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Abstract:

采用基因芯片技术检测了人脐带与骨髓两种不同来源间充质干细胞中miRNA的表达.结果表明:有26个miRNA差异表达,其中miR-29b、miR-20a、miR-31、miR-20b及miR-106a在人脐带间充质干细胞(UMSCs)中表达显著上调,差异倍数均高于3,miR-138则表达下调.在此基础上,采用实时荧光定量PCR技术对差异性显著的6个miRNA进行验证,检测结果一致.最后对特异性基因进行靶点预测,发现靶基因与mRNA转录、翻译以及细胞增殖、分裂、信号转导等功能相关.

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