VORA A C,FITZGERALD M L,GRANDGENETT D P.Removal of 3′-Oh-terminal nucleotides from blunt-ended longterminal repeat termini by the avian retrovirus integration protein[J].Journal of Virology,1990,64(11):5656-5659.
[2]
GREENWALD J,LE V,BUTLER S L,et al.The mobility of an HIV-1 integrase active site loop is correlated with catalyticactivity[J].Biochemistry,1999,38(28):8892-8898.
[3]
GOLDGUR Y,DYDA F,HICKMANAB,et al.Three newstructures of the core domain of HIV-1 integrase:an active sitethat binds magnesium[J].Proceedings of the National Academy of Sciences of the United States of America,1998,95(16):9150-9154.
[4]
BUJACZ G,ALEXANDRATOS J,ZHOULIU Q,et al.The catalytic domain of human immunodeficiency virus integrase:ordered active site in the F185Hmutant[J].Febs Letters,1996,398(2-3):175-178.
[5]
CHIU TK,DAVIES D R.Structure and function of HIV-1 integrase[J].Current Topics in Medicinal Chemistry,2004,4(9):965-977.
[6]
HE HQ,MA X H,LIU B,et al.High-throughput real-time assay based on molecular beacons for HIV-1 integrase 3′-processing reaction[J].Acta Pharmacologica Sinica,2007,28(6):811-817.
[7]
DAVID C A,MIDDLETON T,MONTGOMERY D,et al.Microarray compounds screening(microARCS)to identifyinhibitors of HIV integrase[J].Journal of Biomolecular Screening,2002,7(3):259.
[8]
FARNETC M,HASELTINE W A.Integration of human-immunodeficiency-virus type-1 DNA invitro[J].Proceedings of theNational Academy of Sciences of the United States of America,1990,87(11):4164-4168.
[9]
FAUCI A S.The AIDS epidemic-considerations for the 21st century[J].NewEngland Journal of Medicine,1999,341(14):1046-1050.
[10]
LITTLE S J,HOLTE S,ROUTY J P,et al.Antiretroviral-drug resistance among patients recently infected with HIV[J].NewEngland Journal of Medicine,2002,347(6):385-394.
[11]
GAO K,WONG S,BUSHMANF.Metal binding by the D,DX35E motif of human immunodeficiency virus typeⅠintegrase:selective rescue of Cys substitutions by Mn-2 in vitro[J].Journal of Virology,2004,78(13):6715-6722.
[12]
VINK C,GROENINK M,ELGERSMA Y,et al.Analysis of the junctions between human-immunodeficiency-virus type-1proviral DNA and human DNA[J].Journal of Virology,1990,64(11):5626-5627.
[13]
KATZ R A,SKALKA A M.The retroviral enzymes[J].Annual Review of Biochemistry,1994,63:133-173.
[14]
PEMBERTON I K,BUCKLE M,BUC H.The metal ion-induced cooperative binding of HIV-1 integrase to DNA exhibits amarked preference for Mn(Ⅱ)rather than Mg(Ⅱ)[J].Journal of Biological Chemistry,1996,271(3):1498-1506.
[15]
ROTHM J,SCHWARTZBERG P L,GOFF S P.Structure of the termini of DNA intermediates in the integration of retroviralDNA-dependence on in function and terminal DNA-sequence[J].Cell,1989,58(1):47-54.
[16]
BROWN P O,BOWERMAN B,VARMUS H E,et al.Retroviral integration-structure of the initial covalent product and itsprecursor,and a role for the viral in protein[J].Proceedings of the National Academy of Sciences of the United States ofAmerica,1989,86(8):2525-2529.
[17]
FAURE A,CALMELS C,DESJOBERTC,et al.HIV-1 integrase crosslinked oligomers are active in vitro[J].NucleicAcids Research,2005,33(3):977-986.
[18]
HAZUDA D J,HASTINGS J C,WOLFE A L,et al.A novel assay for the DNA strand-transfer reaction of HIV-1 integrase[J].Nucleic Acids Research,1994,22(6):1121-1122.
[19]
XIA C N,LI HB,LIU F,et al.Synthesis of trans-caffeate analogues and their bioactivities against HIV-1 integrase andcancer cell lines[J].Bioorganic&Medicinal Chemistry Letters,2008,18(24):6553-6557.
[20]
MARCHAND C,ZHANG X C,PAIS G C G,et al.Structural determinants for HIV-1 integrase inhibition by beta-diketoacids[J].Journal of Biological Chemistry,2002,277(15):12596-12603.
[21]
HWANG Y R D,BUSHMAN F.Rapid microtiter assays for provirus topoisomerase,mammalian type IB topoisomerase andHIV-1 integrase:application to inhibitor isolation[J].Nucleic Acids Research,2000,28(24):4884-4892.
[22]
JOHN S,FLETCHER TM,JONSSON C B.Development and application of a high-throughput screening assay for HIV-1integrase enzyme activities[J].Journal of Biomolecular Screening,2005,10(6):606-614.
[23]
HE HQ,MA X H,LIU B,et al.A novel high-throughput format assay for HIV-1 integrase strand transfer reaction usingmagnetic beads[J].Acta Pharmacologica Sinica,2008,29(4):397-404.
[24]
WANG Y,KLOCK H,YINH,et al.Homogeneous high-throughput screening assays for HIV-1 integrase 3′-processing andstrand transfer activities[J].Journal of Biomolecular Screening,2005,10(5):456-462.
[25]
CHEREPANOV P,SURRATT D,TOELEN J,et al.Activity of recombinant HIV-1 integrase on mini-HIV DNA[J].Nucleic Acids Research,1999,27(10):2202-2210.
[26]
HE HQ,LIU B,ZHANG X Y,et al.Development of a high-throughput assay for the HIV-1 integrase disintegrationreaction[J].Science China-Life Sciences,2010,53(2):241-247.
[27]
WAGSTAFF K M,RAWLINSONS M,HEAPRS AC,et al.An alphascreen(R)-based assay for high-throughput screeningfor specific inhibitors of nuclear import[J].Journal of Biomolecular Screening,2011,16(2):192-200.
