全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

蜡状芽孢杆菌的筛选及其产氢性能

Keywords: 蜡状芽孢杆菌,菌种鉴定,产氢性能

Full-Text   Cite this paper   Add to My Lib

Abstract:

为了研究纯菌种对产氢的影响,从污水厂活性污泥中分离了纯的产氢菌种XN12,根据16SrDNA序列和DnaJ基因序列、细胞形态、生理生化数据等多项鉴定分析,该菌种被鉴定为蜡状芽孢杆菌(Bacilluscereus).为了研究该纯菌的产氢性能,实验以葡萄糖为底物,在不同pH值条件下观察其产气情况.实验结果表明:以每摩尔葡萄糖计,该菌在中性条件下获得最大产氢量为1.6mol.对反应的液相产物的检验结果表明:该菌为丁酸发酵菌,通过丙酮酸代谢途径产出氢气.

References

[1]  DAS D. Advances in biohydrogen production processes: an approach towards commercialization[J]. Int J Hydrogen Energ, 2009, 34: 7349-7357.
[2]  黄鹭强. 芽抱杆菌(Bacillus sp. )产脂肪酶条件的优化及酶的纯化、酶学特性的研究[D]. 福州: 福建师范大学生物工程学院, 2004.
[3]  HUANG Lu-qiang. Study on fermentation conditions optimized, purification, characterization of lipase from Bacillus sp. [ D ]. Fuzhou: College of Biological Engineering, Fujian Normal University, 2004. ( in Chinese)
[4]  WANG M Y, TSAI Y L, OLSON B H. Monitoring dark hydrogen mfermentation performance of indigenous Clostridium butyricum by hydrogenase gene expression using RT-PCR and qPCR [ J]. Int J Hydrogen Energ,2008, 33: 4730-4738.
[5]  WANG X, MONIS P T, SAINT C P, et al. Biochemical kinetics of fermentative hydrogen production by Clostridium butyricum W5[J]. Int J Hydrogen Energ, 2009, 34: 791-798.
[6]  TOLVANEN K, KOSKINEN P, RAUSSI H M. Profiling the hydA gene and hydA gene transcript levels of Clostridium butyricum during continuous, mixed-culture hydrogen fermentation[J]. Int J Hydrogen Energ, 2008,33: 5416-5421.
[7]  HEYNDRICKX M, VANSTEENBEECK A, DE VOS P, et al. Hydrogen gas production from continuous fermentation of glucose in a minimal medium with Clostridium butyricum LMG 1213tl[J]. Syst Appl Microbiol, 1986, 8: 239-244.
[8]  张翀. 产气肠杆菌荧光定量追踪及其NADH 产氢途径机理研究[D]. 北京: 清华大学化学工程系, 2007.
[9]  ZHANG Chong. Study on fluorescence-based quantification of Enterobacter aerogenes and its NADH pathway mechanism for hydrogen production [ D ]. Beijing:Chemical Engineering and Technology department of Tsinghua University, 2007. (in Chinese)
[10]  李宇亮, 李小明, 郭亮, 等. 高效产氢菌株Enterococcus sp. LG1 的分离及产氢特性[J]. 微生物学通报, 2008,35(9): 1373-1378.
[11]  LI Yu-liang, LI Xiao-ming, GUO Liang, et al. Isolation and characterization of a new high efficient H2 -producing bacterium Enterococcus sp. LG1 [ J ]. Microbiology,2008, 35(9): 1373-1378. (in Chinese)
[12]  OLSON W P. Automated microbial identification and quantitation: technologies for the 2 000 s [ M ]. Boca Raton: CRC Press, 1996: 13-51.
[13]  KIM D H, KIM S H, SHIN H S. Hydrogen fermentation of food waste without inoculum addition [J]. Enzyme and Microbial Technology, 2009, 45: 181-187.
[14]  HIRASAWA J S, SARTI A, AGUILA N K S D, et al. Application of molecular techniques to evaluate the methanogenic archaea and anaerobic bacteria in the presence of oxygen with different COD: sulfate ratios in a UASB reactor [J]. Anaerobe, 2008, 14: 209-218
[15]  BERTHIER F, EHRLICH S D. Genetic diversity within Lactobacillus sakei and Lactobacillus curvatus and design of PCR primers for its detection using randomly amplified polymorphic DNA [J]. Int J Syst Bacteriol, 1999, 49:997-1007.
[16]  PUGLISI E, VASILEIADIS S, DEMIRIS K, et al. Impact of fungicides on the diversity and function of non-target ammonia-oxidizing microorganisms residing in a litter soil cover [J]. Microb Ecol, 2012, 64: 692-701.
[17]  闫倩. 梭菌氢酶基因hyd 的同源克隆、原核表达以及敲除载体的构建[D]. 西安: 西北农林科技大学生命科学学院, 2012.
[18]  YAN Qian. Homologous cloning, prokaryotic expression and construction of knockout vector of clostridium hydrogenase gene [D]. Xi'an: College of Life Science,Northwest Agriculture and Forestry University, 2012. (in Chinese)
[19]  陈悟, 汪文俊, 向福, 等. 腐蚀生物膜垢中硫酸盐还原菌的系统进化分析[J]. 微生物学通报, 2008, 35(2): 161-165.
[20]  CHEN Wu, WANG Wen-jun, XIANG Fu, et al.Lineages evolution analysis of sulfate-reducing bacteria in the corrosion biofilm[J]. Microbiology, 2008, 35(2):161-165. (in Chinese)
[21]  KHANAL S K, CHEN W H, LI L, et al . Biological hydrogen production: effects of pH and intermediate products[J]. Int J Hydrogen Energ, 2004, 29 (11):1123-1131.
[22]  宗文明, 于瑞嵩, 樊美珍, 等. 一株嗜温高效产氢细菌Clostridium sp. 08-1 的分离鉴定与产氢特征[J].武汉大学学报: 理学版, 2009, 55(5): 569-576.
[23]  ZONG Wen-ming, YU Rui-song, FAN Mei-zhen, et al. Isolation , identification and hydrogen production of Clostridium sp. strain 08-1 , a mesophilic efficient biohydrogen producing bacterium [ J ]. J Wuhan University: Nat Sci Ed, 2009, 55 (5): 569-576. (in Chinese)
[24]  GRAY C T, GEST H. Biological formation of molecular hydrogen production[J]. Science, 1965, 148: 186-192.
[25]  TANISHO S. A Strategy for improving the yield of hydrogen by fermentation [ C]Proceedings of 13th WHEC. In Hydrogen energy progress XIII. [S. l. ]: Int Assoc Hydrogen Energy, 2000: 370-374.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133