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p(C)/p(N)对3BER-S工艺特性及反硝化细菌群落特征的影响

Keywords: 电极生物膜,单质硫,耦合反硝化工艺,碳氮比,反硝化细菌

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Abstract:

为提高三维电极生物膜工艺脱氮效率,通过运行不同TOC与TN的质量浓度比(p(C)/p(N))条件下三维电极生物膜-硫自养耦合工艺(3BER-S),并建立基于反硝化特异性基因nirS克隆文库,研究了p(C)/p(N)对3BER-S运行特性及反硝化细菌群落的影响.结果表明:p(C)/p(N)对3BER-S工艺的脱氮效率影响较小,不同p(C)/p(N)条件下的TN去除效率基本稳定在80%以上.p(C)/p(N)对3BER-S体系内的反硝化细菌种群结构和营养类型均有一定影响.高p(C)/p(N)条件下,反硝化细菌种类较少,Thauera(陶厄氏菌属)是体系内的优势菌群,脱氮作用以异养反硝化过程为主;当p(C)/p(N)降低时,反硝化细菌种类增多,硫自养反硝化细菌所占比例升高.总之,由于硫磺单质的加入,弥补了3BER工艺低p(C)/p(N)时的反硝化作用电子供体不足,使得3BER-S耦合体系在不同p(C)/p(N)条件下均能保持高效且稳定的脱氮效果.

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