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高效脱硫菌和甲苯降解菌的筛选、组合及其降解条件的优化

Keywords: 脱硫菌,甲苯降解菌,筛选,优化组合,响应面法

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Abstract:

以脱硫菌和甲苯降解菌为研究对象,分别以硫代硫酸钠和甲苯为驯化底物,研究了2类菌种的驯化、筛选、组合及混合菌的降解条件.通过驯化菌源,筛选得到高效脱硫菌4株(Z1、Z2、Z3、B1),甲苯降解菌3株(J1、J2、J3),并优化组合2类菌种得到混合菌的最佳组合方式为(Z1+Z2)-B1-(J1+J3),且其菌体密度组合比例为2∶4∶3.同时以SO2-4的质量浓度和甲苯降解率为控制指标,采用响应面法的中心组合设计(centralcompositedesign,CCD)优化混合菌的降解条件,分析得到混合菌的最优降解条件为:pH6.6,温度30.0℃,接种量3.0%,甲苯添加量3.0μL,硫代硫酸钠加入量4.0g/L.验证实验表明,在最优条件下混合菌的甲苯降解率为73.9%,培养液中SO2-4的质量浓度为163.9mg/L,基本与预测结果一致,较优化前有明显提高.

References

[1]  PARK J,EVANS E A,ELLIS T G.Development of a biofilter with tire-derived rubber particle media for hydrogen sulfide odor removal[J].Water Air and Soil Pollution,2011,215(1/2/3/4):145-153.
[2]  王丽燕,王爱杰,任南琪,等.有机废气(VOC)生物处理研究现状与发展趋势[J].哈尔滨工业大学学报,2004,36(6):732-735.WANG Li-yan,WANG Ai-jie,REN Nan-qi,et al.Present status and trend of bio-treatment of volatile organic compound[J].Journal of Harbin Institute of Technology,2004,36(6):732-735.(in Chinese)
[3]  REZA I,HUUB H J C,MARC A D.Literature review of air pollution control biofilters and biotrickling filters for odor and volatile organic compound removal[J].Environmental Progress,2005,24(3):255-267.
[4]  NISOLA G M,TUUGUU E,FARNAZO D M D,et al.Hydrogen sulfide degradation characteristics of Bordetella sp.Sulf-8 in a biotrickling filter[J].Bioprocess and Biosystems Engineering,2010,33(9):1131-1138.
[5]  RAMIREZ M,GOMEZ J M,AROCA G,et al.Removal of hydrogen sulfide by immobilized Thiobacillus thioparus in a biotrickling filter packed with polyurethane foam[J].Bio-resource Technology,2009,100:4989-4995.
[6]  LEE E Y,LEE N Y,CHO K S,et al.Removal of hydrogen sulfide by sulfate-resistant Acidithiobacillus thiooxidans AZ11[J].Journal of Bioscience and Bioengineering,2006,101(4):309-314.
[7]  LU Y,LIU J,LU B,et al.Study on the removal of indoor VOCs using biotechnology[J].Journal of Hazardous Materials,2010,182(1/2/3):204-209.
[8]  彭淑婧,李坚,刘佳,等.静压箱式错流生物滴滤床去除甲苯废气的研究[J].环境污染与防治,2011,33(12):37-41.PENG Shu-jing,LI Jian,LIU Jia,et al.Purification of toluene contained waste gas by static pressure tank crossflow bio-trickling filter[J].Environmental Pollution&Control,2010,33(12):37-41.(in Chinese)
[9]  AKHNAZARVA S,KEFAROV V.Experiment optimization in chemistry and chemical engineering[M].Moscow:Mir Publishers,1982:57-89.
[10]  KHURI A I,CORNELL J A.Response surfaces:design and analysis[M].New York:Marcel Dekker Inc,1987:132-146.
[11]  VOHRA A,SATYANARAYANA T.Statistical optimization of the medium components by response surface methodology to enhance phytase production by Pichia anomala[J].Process Biochem,2002,37(9):999-1004.

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