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城市污水SBR法短程生物脱氮系统硝化菌群的定量分析

Keywords: SBR,短程生物脱氮,Fish,PCR-DGGE,PCR-克隆序列

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Abstract:

为了研究实时控制对短程生物脱氮中试系统内硝化菌群结构组成的影响,在考察系统常温条件下处理城市污水时短程硝化效果的同时,采用FISH、PCR-DGGE和PCR-克隆序列(CloningSequencing)分子生物学方法对SBR中试系统(有效容积为54m~3)硝化菌群中的氨氧化菌(AOB)和亚硝酸氧化菌(NOB)进行定性与定量化分析.FISH结果表明,在短程脱氮系统中,AOB相比于NOB已成为明显的优势菌群,占总菌群的3%~12%;且没有检测出NOB.PCR-DGGE结果表明,SBR短程脱氮中试系统中的AOB均以Nitrosomonas-like为主.污泥样品的PCR-Cloning-Sequencing结果表明,所有的克隆相似于Nitrosomonas,其中60%以上的克隆相似于Nitro-somonaseuropaea.

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