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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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蛋白质磷酸化作用的电化学检测方法
DOI: 10.7536/PC131056, PP. 856-865
Keywords: 磷酸化作用,电化学检测,修饰电极
Abstract:
蛋白质的磷酸化作用是一种可逆的翻译后修饰方式，几乎参与生物体内所有关键生命活动，在核内基因的特定表达、细胞的分裂分化以及各种生命活动的转导中发挥重要意义。基于磷酸化前后电化学信号的改变，利用电化学传感器的方法能快速简便地检测蛋白质的磷酸化作用，且具有较高的选择性和灵敏度。本文回顾了几种常用的电化学检测磷酸化作用的方法，基于电极材料的不同进行了分类与总结，汇总了每种方法中用到的修饰电极的纳米材料及生物分子，最后总结了电化学方法检测蛋白质磷酸化的优缺点，并对有效检测磷酸化作用进行了前景展望。
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