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OALib Journal期刊
ISSN: 2333-9721
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检测硫化氢分子的荧光探针
DOI: 10.7536/PC131155, PP. 1065-1078
Keywords: 硫化氢,荧光探针,活性硫物种,气体递质,可视化检测
Abstract:
硫化氢（H2S）是继一氧化碳和一氧化氮之后，第三种可在生命体内发挥生理作用的内源性气体信号分子。该气体分子在心血管和神经系统中担负着重要的生理病理调节作用。因此，选择性识别和高灵敏检测生物体内的H2S具有十分重要的生物医学意义。在生物检测技术手段中，荧光探针法具有选择性好、灵敏度高、对生物样品损伤小以及可实现实时原位检测等独特的优势，故应用荧光探针法检测细胞内H2S浓度的变化是近年来研究热点之一。本文依据荧光探针与H2S之间的化学反应类型，将近三年来所研发的H2S荧光探针按照其母体荧光团进行分类和总结，综述了H2S荧光探针的研究进展，概述了相关荧光探针的设计理念、检测机理及生物应用，探讨了探针的结构和性能之间的关系，最后展望了H2S荧光探针的发展趋势和应用前景。
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