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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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亲水作用色谱固定相的发展及应用
DOI: 10.7536/PC130661, PP. 10-18
Keywords: 亲水作用色谱,固定相,极性药物,蛋白质组学,代谢组学
Abstract:
亲水作用色谱作为一种高效液相色谱技术，在强极性和离子型化合物如氨基酸、碳水化合物和多肽等的分离分析中发挥着重要作用。作为色谱技术的核心，亲水作用色谱材料的发展直接影响着色谱分离的选择性和分离效率，制约着亲水作用色谱的应用和推广。目前商品化和学术报道的亲水作用色谱材料种类繁多，键合相结构丰富。本文从色谱材料结构出发，综述了近5年内基于硅胶基质的亲水作用色谱固定相，包括纯硅胶、氨基、氰基、二醇基、酰胺型、聚（琥珀酰亚胺）型、糖型和两性离子型键合相的发展及其在极性药物、蛋白质组学、代谢组学等方面的典型应用。同时简要介绍了近年来亲水作用色谱的色谱评价研究。
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