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OALib Journal期刊
ISSN: 2333-9721
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PNIPAAm改性表面对蛋白质吸附的调控及其应用
DOI: 10.7536/PC140350, PP. 1275-1284
Keywords: 聚（N-异丙基丙烯酰胺）,表面改性,蛋白质吸附,温度敏感性
Abstract:
根据不同领域的需要，控制蛋白质在材料表面的吸附是一个具有重要应用价值的课题。聚（N-异丙基丙烯酰胺）（PNIPAAm）改性表面能够响应外界温度变化从而改变其表面性质，这一特点为调控蛋白质的吸附提供了可能。近年来，研究者们应用多种表征方法考察了不同温度下蛋白质在PNIPAAm改性表面的吸附，并试图从分子水平上深入理解其吸附机制及影响因素。本文综述了近年来应用PNIPAAm改性表面对蛋白质吸附的研究及其最新进展。发现当PNIPAAm层厚度处于一定范围内时，PNIPAAm改性表面表现出对蛋白质吸附的温度敏感性，并可以利用这一性质将其应用于蛋白质纯化及分离和生物传感器等领域。而当PNIPAAm层厚度超过一个临界值时，PNIPAAm改性表面表现出良好的阻抗血浆蛋白质的性质，使其有望在血液相容性表面领域得到应用。最后，就PNIPAAm改性表面调控蛋白质吸附的未来发展方向简要地进行了展望。
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