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化学进展  2014 

纤维素类手性色谱固定相的制备及其应用

DOI: 10.7536/PC130776, PP. 415-423

Keywords: 纤维素衍生物,手性固定相,超临界流体色谱,模拟移动床色谱

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Abstract:

对映体的分离、分析及检测是目前医药与生化领域研究的热点之一。使用手性固定相并通过高效液相色谱直接拆分手性消旋体是现行单一对映体获取最直接且有效的手段之一，其中长链多糖类化合物如纤维素衍生物具有较强的手性识别能力，以此作为手性识别体的手性色谱固定相占有广阔的市场。本文对现有纤维素类手性色谱固定相的制备方法加以分类，评述了近年来该类别涂敷型、键合型及杂化型手性固定相的制备新方法，总结了采用衰减全反射傅里叶变换红外光谱技术、X射线衍射、固体核磁、密度泛函理论模拟等手段研究其手性识别机理的研究进展，综述了其在制备型超临界流体色谱和模拟移动床色谱中的应用情况。今后，对纤维素手性固定相的研究将主要集中在改善手性识别性能、提高样品处理能力以及降低溶剂消耗等方面，因此新型手性识别体的开发和负载方式的优化革新将是新一代手性固定相的研究关键，同时，应用领域的拓宽也将是其重要的发展方向之一。

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