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OALib Journal期刊
ISSN: 2333-9721
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用于毛细管电泳DNA分离的合成聚合物*
Keywords: DNA分离,毛细管电泳,无胶筛分,聚合物溶液,合成水溶性聚合物
Abstract:
毛细管电泳的无胶筛分方法在DNA片段分离、DNA测序方面取得了显著的成绩并已成功应用于人类基因组计划.该法是在毛细管柱中充入一定浓度和组成的线性高分子溶液,利用其对样品组分电泳迁移时的阻滞作用,按分子量大小对DNA等生物大分子进行筛分分离分析.因此,聚合物筛分介质的类型、组成和性质会显著影响分离效果.近年来,由于受到基因组计划的影响,出现了许多用于DNA片段分离和DNA测序的水溶性高分子聚合物,并取得很大进展.本文按照均聚物和共聚物的分类,综述了作为筛分介质的各种合成聚合物及其应用效果,并简要介绍了有关的筛分理论和分离的评价指标.
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