全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

中药保肝解毒汤对实验性铜中毒欧鳗鳃、肝、肾组织中Na~+-K~+-ATPase活性的影响

Keywords: 保肝解毒汤,欧鳗,硫酸铜,实验性中毒,Na+-K+-ATPase活性

Full-Text   Cite this paper   Add to My Lib

Abstract:

应用生物化学方法研究了由茵陈、龙胆、甘草、大黄及栀子组成的中药保肝解毒汤对铜中毒欧鳗鳃、肝、肾组织中Na+-K+-ATPase活性的影响。健康组欧鳗养于清水中,4个处理组先暴露于0.12mg.L-1Cu2+溶液4d,然后以其中一组作为阳性空白对照组不治疗,其余3组为中药治疗组,即保肝解毒汤治疗低剂量组(200mg.L-1)、保肝解毒汤治疗高剂量组(400mg.L-1)和降酶灵(所含成分为五味子)治疗对照组(100mg.L-1)。结果显示(:1)健康欧鳗鳃、肾组织中Na+-K+-ATPase活性分别是肝的3.61倍和3.50倍;(2)铜中毒可致鳃、肝、肾组织中Na+-K+-ATPase活性分别下降5.09%、85.98%和30.15%;(3)保肝解毒汤对铜中毒欧鳗肝组织中Na+-K+-ATPase活性的恢复效果最明显,肾组织次之,鳃组织则效果不明显;(4)3个治疗组中以治疗低剂量组和治疗对照组升高Na+-K+-ATPase活性的效果最为显著,治疗高剂量组效果较差。表明中药保肝解毒汤对铜中毒欧鳗肝、肾组织中Na+-K+-ATPase活性具有恢复作用,但剂量对其药效有影响。

References

[1]  Paris-Palacios S,Biagianti-Risbourg S.Hepatocyte nuclear structure and subcellular distribution of copper in zebrafish Brachydanio rerio and roach Rutilus rutilus (Teleostei,Cyprinidae) exposed to copper sulphate[J].Aquatic Toxicology,2006,77(3):306-313.
[2]  赵健雄,吕晓云,王学习,等.扶正解毒颗粒改善镍暴露者肝酶活性的研究[J].中国职业医学,2003,30(2):27-29.
[3]  朱玉真,赵健雄,王学习,等.中药防治硫酸镍对Ca2+-ATP酶及钙调素活性抑制的实验研究[J].中国职业医学,2001,28(5):21-22.
[4]  Mecocci P,Cherubini A,Beal M F,et al.Altered mitochondria membrane fluidity in AD brain[J].Ne urosci Lett,1996,207:129-139.
[5]  李亚,王建军,陈启盛.β淀粉样蛋白对D半乳糖致衰大鼠海马线粒体膜流动性的影响[J].中国病理生理杂志,2002,12:73-75.
[6]  胡小玲,汪晖,吴基良.阿魏酸钠的抗肝损伤作用及其机制研究[J].中国临床药理学与治疗学,2004,9(1):74-76.
[7]  Claire D,Paris-Palacios S,Betoulle S,et al.Modulation in hepatic and head kidney parameters of carp (Cyprinus carpio L.) induced by copper and chitosan[J].Comparative Biochemistry and Physiology(Part C:Toxicology & Pharmacology),2004,137(4):325-333.
[8]  Varanka Z,Rojik I,Varanka I,et al.Biochemical and morphological changes in carp (Cyprinus carpio L.)liver following exposure to copper sulfate and tannic acid[J].Comparative Biochemistry and Physiology (Part C:Toxicology & Pharmacology),2001,128(3):467-478.
[9]  Gagnon A,Jumarie C,Hontela A.Effects of Cu on plasma cortisol and cortisol secretion by adrenocortical cells of rainbow trout (Oncorhynchus mykiss)[J].Aquatic Toxicology,2006,78(1):59-65.
[10]  陈明家,王宗元,朱模忠,等.鲫鱼实验性铜中毒及治疗的研究[J].水产养殖,1993,1:15-18.
[11]  王安利,母学全,凌利英,等.维生素B12对中国对虾抗Cu(Ⅱ)毒能力的影响[J].河北大学学报(自然科学版),1994,14(2):76-79.
[12]  Ahmad I,Oliveira M,Pacheco M,et al.A nguilla anguilla L.oxidative stress biomarkers responses to copper exposure with or without β-naphthoflavone pre-exposure[J].Chemosphere,2005,61(2):267-275.
[13]  鲁双庆,刘少军,刘筠,等.Cu2+对黄鳝肝脏和性腺组织及其线粒体中(Na++K+)-ATPase活性的影响[J].农业环境保护,2002,21(6):508-511.
[14]  宋稳成,杨仁斌,郭正元.二氯喹啉酸对湘云鲫鳃肝脏ATPase活性的影响及其机制探讨[J].农业环境科学学报,2004,23(1):190-193.
[15]  方展强,王春凤,卫焕荣.汞和硒对剑尾鱼Na+/K+-ATPase活性的影响[J].应用与环境生物学报,2006,12(2):220-223.
[16]  Sancho E,Fernández-Vega C,Ferrando MD,et al.Eel ATPase activity as biomarker of thiobencarb exposure[J].Ecotoxicology and Environmental Safety,2003,56(3):434-441.
[17]  Van der Oost R,Beyer J,Vermeulen N P E.Fish bioaccumulation and biomarkers in environmental risk assessment:a review[J].Environmental Toxicology and Pharmacology,2003,13(2):57-149.
[18]  梅景良,黄一帆,陈文列.中药制剂对欧鳗实验性肝病保护作用的超微病理学研究[J].中国农学通报,2006,22(6):468-474.
[19]  陈佳荣.水化学[M].北京:中国农业出版社,1998.260-261.
[20]  黄玉瑶,赵忠宪,陈锦萍,等.铜离子对鳗鲡(Anguilla japonica Temm.et Schl.)仔鱼的急性毒性[J].中国环境科学,1992,12(4):255-260.
[21]  陈奇.中药药理研究方法学[M].北京:人民卫生出版社,1993.112-115.
[22]  Grosell M,Nielsen C,Bianchini A.Sodium turnover rate determines sensitivity to acute copper and silver exposure in freshwater animals[J].Comparative Biochemistry and Physiology,2002,133(C):287-303.
[23]  袁锦芳,陈叙龙,张毓琪.环境因素对海洋动物Na+-K+-ATPase的影响概述[J].海洋环境科学,1999,18(3):75-79.
[24]  孔祥会,王桂忠,李少菁,等.锯缘青蟹不同器官组织中4种类型ATPase活性比较研究[J].厦门大学学报(自然科学版),2004,43(1):98-101.
[25]  Lionetto M G,Maffia M,Cappello M S,et al.Effect of cadmium on carbonic anhydrase and Na+-K+-ATPase in eel,Anguilla anguilla,intestine and gills[J].Comparative Biochemistry and Physiology,1998,120(A):89-91.
[26]  刘福军,张饮江,王明学.铜对鱼类慢性毒性研究进展[J].水生生物学报,2003,27(3):302-307.
[27]  Marr J C A,Lipton J,Cacela D,et al.Relationship between copper exposure duration,tissue copper concentration,and rainbow trout growth[J].Aquat Toxicol,1996,36:17-30.
[28]  Giguère A,Campbell P G C,Hare L,et al.Influence of lake chemistry and fish age on cadmium,copper,and zinc concentrations in various organs of indigenous yellow perch (Perca flavescens)[J].Canadian Journal of Fisheries and Aquatic Sciences,2004,61(9):1702-1716.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133