全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

不同水分条件下不同土壤微生物类群产N_2O量的初步研究

Keywords: 自养硝化,异养硝化/好氧反硝化,厌氧反硝化,土壤充水孔隙度,N2O产生

Full-Text   Cite this paper   Add to My Lib

Abstract:

本研究主要是通过微生物分离手段加富培养菜园土中的硝化菌、厌氧反硝化菌和异养硝化/好氧反硝化菌,并在30%、50%和100%3种土壤充水孔隙度(指土壤体积含水量与总孔隙度的百分比,V/V)水分条件下进行无菌菜园土反接种培养实验,研究了不同水分条件下、不同土壤微生物类群的产N2O量。结果表明:30%含水量的灭菌土壤反接种培养,N2O的产生是以异养硝化/好氧反硝化作用为主,自养硝化作用对于N2O亦有贡献,后者产N2O量仅是前者的15.2%~47.2%;而50%含水量的灭菌土壤反接种培养,N2O的产生过程是以自养硝化为主,其N2O产生量几乎是异养硝化/好氧反硝化菌处理的2.1倍。在上述3种水分条件下,厌氧反硝化菌活性均受到抑制,几乎不产生N2O。

References

[1]  Ghani A,Dexter M,Perrott K W.Hot-water extractable carbon in soils:a sensitive measurement for determining impacts of fertilization,grazing and cultivation[J].Soil Biology & Biochemistry,2003,35:1231-1243.
[2]  IPCC,Climate Change 2001.(http://www.ipcc.ch/)
[3]  Delgado J A,Mosier A R.Mitigation alternatives to decrease nitrous oxides emissions and urea-nitrogen loss and their effect on methane flux[J].Journal of Environmental Quality,1996,25(5):1105-1111.
[4]  Rover M,Heinemeyer O,Kaiser E.Microbial induced nitrous oxide emissions from an arable soil during winter[J].Soil Biology & Biochemistry,1998,30:1859-1865.
[5]  Robertson L A,Kuenen J G.Aerobic denitrification-old wine in new bottles[J].A ntonievan Leeuwenhoek,1984,50:525-544.
[6]  Anderson I C,Poth M,Homstead J,et al.A comparison of NO and N2O production by the autotrophic nitrifier Nitrosomonas europaea and the heterotrophic nitrifier Alcaligenes faecalis[J].Applied and Environmental Microbiology,1993,59(11):3525-3533.
[7]  Wolf I,Brumme R.Contribution of nitrification and denitrification sources for seasonal N2O emissions in an acid German forest soil[J].Soil Biology & Biochemistry,2002,34:741-744.
[8]  杜睿,王庚辰,吕达仁.内蒙古典型草原土壤N2O产生的机理探讨[J].中国环境科学,2000,20(5):387-391.
[9]  Matsuzaka E,Nomura N,Nakajima-Kambe T,et al.A Sample Screening Procedure for Heterotrophic Nitrifying Bacteria with Oxygen-Tolerant Denitrification Activity[J].Journal of Bioscience and Bioengineering,2003,95(4):409-411.
[10]  郑循华,王明星,王跃思,等.稻麦轮作生态系统中土壤湿度对N2O产生与排放的影响[J].应用生态学报,1996,7(3):273-279.
[11]  Bateman E J,Baggs E M.Contributions of nitrification and denitrification to N2O emissions from soils at different water-filled pore space[J].Biology and Fertility of Soils,2005,3 (http://springer.lib.tsinghua.edu.cn/).
[12]  McTaggart I P,Akiyama H,Tsuruta H,et al.Influence of soil physical properties,fertiliser type and moisture tension on N2O and NO emissions from nearly saturated Japanese upland soils[J].Nutrient Cycling in Agroecosystems,2002,63:207-217.
[13]  Product Information Sheet For ATCC 25196.(http://www.atcc.org/)
[14]  李阜棣,喻自牛.农业微生物学实验技术[M].北京:中国农业出版社,1996.308.
[15]  Stevenson B A,Verburg P S J.Effluxed CO2-13C from sterilized and unsterilized treatments of a calcareous soil[J].Soil Biology & Biochemistry,2006,38:1727-1733.
[16]  Walker N,编.中国科学院南京土壤研究所微生物室,译.土壤微生物学[M].北京:科学出版社,1981.223-248.
[17]  陈文新.土壤和环境微生物学[M].北京:北京农业大学出版社,1989.139-147.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133