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铆钉菇对重金属的耐性及其对油松分泌TOC的影响

Keywords: 铆钉菇,Cu,Cd,pH,TOC

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Abstract:

为了进一步了解外生菌根真菌抗重金属胁迫机理,采用室内培养方法,研究了在不同pH环境下,纯培养铆钉菇的生长状况和培养环境的变化。结果表明,pH是影响菌根真菌抗重金属能力的重要因素。对照和Cd50μmol·L-1处理下,菌根真菌最佳生长pH条件为5.0;Cu50μmol·L-1处理和Cu25μmol·L-1+Cd25μmol·L-1混合处理下,菌根真菌最佳生长pH条件为4.0。菌根真菌能够通过调节自身的培养环境来缓解过高或过低pH的胁迫作用,使培养体系pH向适于真菌生长的方向改变。这种调节作用在重金属处理条件下更加明显。在此基础上,研究了铆钉菇和油松共生条件下,重金属处理前后菌根分泌物总有机碳含量(TotalOrganicCarbon)的变化。结果表明,在Cu处理后,接种G.viscidus油松分泌物的TOC含量显著高于Cu处理前,说明菌根真菌可以通过分泌有机物质来螯合、固定重金属,影响重金属的生物有效性,从而达到缓解胁迫的目的。

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